Optimization of Extraction and Purification Process for Xiyue Granules

XIA Hua-ling; DU Zhi-qian

doi:10.13422/j.cnki.syfjx.2014210009

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Optimization of Extraction and Purification Process for Xiyue Granules

更新时间：2024-01-04

- Optimization of Extraction and Purification Process for Xiyue Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 9-12(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210009
  CLC： R283.6;R284.2
- Published：2014
- 稿件说明：
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XIA Hua-ling, DU Zhi-qian. Optimization of Extraction and Purification Process for Xiyue Granules[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 9-12.
DOI：

XIA Hua-ling, DU Zhi-qian. Optimization of Extraction and Purification Process for Xiyue Granules[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 9-12. DOI： 10.13422/j.cnki.syfjx.2014210009.

摘要

目的: 优选膝悦颗粒的提取及纯化工艺。方法: 采用HPLC-ELSD测定黄芪甲苷含量

流动相乙腈-水(35:65);采用HPLC测定龙胆苦苷含量

流动相甲醇-水(27:73)

流速0.8 mL·min-1

检测波长271 nm。以黄芪甲苷和龙胆苦苷含量的综合评分为指标

选择加水量、提取时间、提取次数为考察因素

采用正交试验优选膝悦颗粒的提取工艺;以黄芪甲苷、龙胆苦苷含量及出膏率为评价指标

通过单因素试验筛选膝悦颗粒的纯化工艺。结果: 最佳提取工艺为加10倍量水煎煮3次

每次1 h;黄芪甲苷、龙胆苦苷质量分别为4.34

43.7 mg。采用醇沉法

使含醇量达50%;黄芪甲苷21.30 mg(保留率85.61%)

龙胆苦苷216.32 mg(保留率97.74%)

出膏率22.13%。结论: 优选的提取及纯化工艺稳定可行

为膝悦颗粒的工业化生产提供参考。

Abstract

Objective: To optimize extraction and purification procedures of Xiyue granules. Method: HPLC-ELSD was employed to determine the content of astragaloside IV with mobile phase of acetonitrile-water(35:65). HPLC was adopted to determine the content of gentiopicroside by taking methanol-water(27:73) as mobile phase

flow rate was 0.8 mL·min-1 and detection wavelength was 271 nm.Taking composite score of contents of astragaloside IV and gentiopicroside as index

effects of the amount of water

extracting time and times on extraction process was optimized by orthogonal design.Single factor tests were adopted to optimize purification procedure with dry extract rate and contents of astragaloside IV and gentiopicroside as indexes. Result: The best extraction process was as follows:extracted 3 times with 10 times the amount of water for 1.0 h each time;contents of astragaloside IV and gentiopicroside were 4.34

43.7 mg

respectively.Optimum purification process was ethanol precipitation which contained 50% alcohol;contents of astragaloside IV and gentiopicroside

dry extract rate were 21.30 mg

216.32 mg and 22.13%. Conclusion: Optimized extraction and purification processes are stable and reliable

they can provide references for industrial production of Xiyue granules.

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