Improvement of Extraction Method of DNA from Asini Corii Colla

CHEN Zhi-xuan; GONG Guo-li; QIAN Yun-kai

doi:10.13422/j.cnki.syfjx.2014210013

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Improvement of Extraction Method of DNA from Asini Corii Colla

更新时间：2024-01-04

- Improvement of Extraction Method of DNA from Asini Corii Colla
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 13-16(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210013
  CLC： R283.6;R284.2;R282.74
- Published：2014
- 稿件说明：
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CHEN Zhi-xuan, GONG Guo-li, QIAN Yun-kai. Improvement of Extraction Method of DNA from Asini Corii Colla[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 13-16.
DOI：

CHEN Zhi-xuan, GONG Guo-li, QIAN Yun-kai. Improvement of Extraction Method of DNA from Asini Corii Colla[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 13-16. DOI： 10.13422/j.cnki.syfjx.2014210013.

摘要

目的: 优选阿胶DNA的提取方法和效率

为其他高蛋白含量产品中DNA的提取提供参考。方法: 采用十二烷基苯磺酸钠(SDS)法和十六烷基三甲基溴化铵(CTAB)法提取阿胶DNA

针对阿胶产品的自身特性

优选SDS法中蛋白酶K作用时间和蛋白洗脱步骤。结果: 在同等条件下

SDS法较CTAB法对阿胶DNA的平均提取量高0.022 g·L-1。优化的SDS法为蛋白酶K作用时间1.5 h

使用酚-三氯甲烷-异戊醇洗脱阿胶中蛋白质

重复3次;阿胶DNA的纯度较原工艺提高了48.3%。结论: 采用SDS法对阿胶中蛋白的清除作用较CTAB法效率高

优化的SDS法对阿胶DNA的提取适用性较强

为阿胶中动物来源分子生物学检测提供条件。

Abstract

Objective: To improve extraction efficiency of DNA in Asini Corii Colla and provide a reference for extracting DNA in other products containing high protein content. Method: DNA in Asini Corii Colla was extracted by SDS method and CTAB method

according to their own characteristics of Asini Corii Colla products

reaction time of proteinase K and protein elution step in SDS method were optimized. Result: Under the same conditions

average extraction capacity of DNA in Asini Corii Colla by SDS was higher than CTAB

whose numerical difference was 0.022 g·L-1.Optimized SDS method was as following:reaction time of proteinase K 1.5 h

eluted protein in Asini Corii Colla by phenol-chloroform-isoamyl alcohol for three times

purity of DNA in Asini Corii Colla increased 48.3% by comparing with the original process. Conclusion: Scavenging effects on protein in Asini Corii Colla by SDS method is higher than CTAB

this optimized SDS method is suitable for extraction of DNA in Asini Corii Colla

it provides conditions for biological detection of animal derived molecules from Asini Corii Colla.

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Related Author

Jia-chen ZHAO

Zhi-lai ZHAN

Hua-min ZHANG

Hong-jun YANG

Bing LI

Qiu-jie CAI

Hua-sheng PENG

Wei ZHANG

Related Institution

National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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