Investigation on Extraction and Antioxidant Activity of Total Polyphenols from Seeds of

LI Guo-jin; GENG Hong-mei; ZANG Wei

doi:10.13422/j.cnki.syfjx.2014210028

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Investigation on Extraction and Antioxidant Activity of Total Polyphenols from Seeds of

更新时间：2024-01-04

- Investigation on Extraction and Antioxidant Activity of Total Polyphenols from Seeds of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 28-30(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210028
  CLC： R283.6;R284.2;R285.5
- Published：2014
- 稿件说明：
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LI Guo-jin, GENG Hong-mei, ZANG Wei. Investigation on Extraction and Antioxidant Activity of Total Polyphenols from Seeds of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 28-30.
DOI：

LI Guo-jin, GENG Hong-mei, ZANG Wei. Investigation on Extraction and Antioxidant Activity of Total Polyphenols from Seeds of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 28-30. DOI： 10.13422/j.cnki.syfjx.2014210028.

摘要

目的: 优选白花菜子总多酚的提取工艺并初步探讨其抗氧化活性。方法: 采用UV测定总多酚含量

检测波长760 nm。以总多酚提取量为指标

选择料液比、提取温度、乙醇体积分数、提取时间为考察因素

通过单因素试验和正交试验优选白花菜子总多酚的提取工艺条件

考察其对二苯代苦味酰基自由基(DPPH·)的清除能力。结果: 最佳提取工艺为加20倍量70% 乙醇于50 ℃超声提取40 min;总多酚提取量1.93 mg·g-1。抗氧化活性试验表明当白花菜子总多酚提取液质量浓度为1.28 g·L-1时

清除DPPH·自由基的能力与抗坏血酸抗氧化能力相近

均>96%。结论: 优选的超声提取工艺稳定可行

白花菜子总多酚具有较强的抗氧化活性

为白花菜子资源的开发利用提供实验依据。

Abstract

Objective: To optimize extraction technology of total polyphenols from seeds of Cleome gynandra and evaluate its antioxidant activities. Method: UV was adopted to determine the content of total polyphenols with detection wavelength at 760 nm.Taking the content of total polyphenols as index

selecting solid-liquid ratio

extracting temperature

solvent concentration

extracting time as factors

extraction conditions of total polyphenols were optimized by single factor tests and orthogonal test

its scavenge activity on DPPH·free radical was discussed. Result: The best extraction technology was as follows:extracted 40 min with 20 times the amount of 70% ethanol;the content of total polyphenols was 1.93 mg·g-1.Antioxidative activity test showed that DPPH·scavenging abilities of total polyphenols extract from seeds of C. gynandra and vitamin C had no difference when its concentration of 1.28 g·L-1

they were more than 96%. Conclusion: This optimized ultrasonic extraction technology is stable and feasible

total polyphenols have strong antioxidant activity

this article can provide experimental basis for development and utilization of seeds of C. gynandra.

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