Optimization of Extraction Process of Effective Fraction from Garidi Powders by Orthogonal Test

PEI Ling-yan; CUI Jian; LIU Wei-zhi; QIN A-na; WANG Han-cong; WANG Meng-meng; Borjigidai Almaz; ZHU Dan

doi:10.13422/j.cnki.syfjx.2014210031

您当前的位置：

首页 >

文章列表页 >

Optimization of Extraction Process of Effective Fraction from Garidi Powders by Orthogonal Test

更新时间：2024-01-04

- Optimization of Extraction Process of Effective Fraction from Garidi Powders by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 31-34(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210031
  CLC： R287;R283.6;R284.2
- Published：2014
- 稿件说明：
移动端阅览
PEI Ling-yan, CUI Jian, LIU Wei-zhi, et al. Optimization of Extraction Process of Effective Fraction from Garidi Powders by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 31-34.
DOI：

PEI Ling-yan, CUI Jian, LIU Wei-zhi, et al. Optimization of Extraction Process of Effective Fraction from Garidi Powders by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 31-34. DOI： 10.13422/j.cnki.syfjx.2014210031.

摘要

目的: 优选嘎日迪散有效部位的提取工艺

为后期中试生产提供实验依据。方法: 采用非水滴定法测定总生物碱含量;利用UV测定总黄酮含量

检测波长500 nm。以嘎日迪散中总生物碱和总黄酮质量分数为指标

通过正交试验考察乙醇体积分数、溶剂用量、回流时间及提取次数对提取工艺的影响

利用TLC和LC-MS/MS确定提取物中部分生物碱类成分。结果: 影响总黄酮提取率的因素主次顺序为回流时间>溶剂用量>乙醇体积分数>提取次数

最佳提取条件组合为加22倍量70% 乙醇提取5 h;影响总生物碱提取率的因素主次排序为乙醇体积分数>回流时间>溶剂用量>提取次数

最佳提取条件组合为加26倍量80% 乙醇提取4 h;总生物碱和总黄酮质量分数分别为0.139%

0.465%。结论: 根据生产实际条件和成本考虑

确定嘎日迪散最佳提取工艺为加22倍量70% 乙醇回流提取5 h。野罂粟中主要生物碱类成分为野罂粟碱、黑龙辛甲醚、瑞芙热米定和黑龙辛。

Abstract

Objective: To optimize extraction process of effective fraction in Garidi powders and provide experimental evidence for pilot production at later stage. Method: The content of total alkaloids was determined by non-aqueous titration;UV was adopted to determine the content of total flavonoids with detection wavelength at 500 nm.Taking contents of total alkaloids and total flavonoids as indexes

orthogonal test was used to optimize extraction process with ethanol concentration

solid-liquid ratio

extracting time and times as factors

TLC and LC-MS were employed to confirm alkaloids types in extract. Result: Influencing factors acting on yield of total flavonoids was in the order of reflux time > solid-liquid ratio> ethanol concentration > extraction times

optimum extraction conditions of total flavonoids was:extracted 5 h with 22 times the amount of 70% ethanol.Factors acted yield of total alkaloids by ethanol concentration>reflux time>solid-liquid ratio>extraction times

optimum extraction conditions was as followings:extracted 4 h with 26 times the volume of 80% ethanol;mass fractions of total alkaloids and total flavonoids were 0.139% and 0.465%

respectively. Conclusion: According to actual conditions of production and cost

optimum extraction conditions of Garidi powders is as following:reflux extract 5 h with 22 times the volume of 70% ethanol.Main compositions of alkaloids in Papaver nudicaule are nudicauline

amurensinine

reframidine and amurensine.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Nano Oral Colon Targeted Drug Delivery System in Treatment of Ulcerative Colitis： A Review

Comparison of Pharmacokinetics and Tissue Distribution of Free Anthraquinones and Gallic Acid in Mice with Ulcerative Colitis Before and After Processing of Rhei Radix et Rhizoma Carbonisata

Mechanisms of Zhuyuwan in Treating both Intrahepatic Cholestasis and Ulcerative Colitis Based on Homotherapy for Heteropathy

Shaoyaotang Containing Serum Mediates Fas/FasL Pathway to Inhibit Lipopolysaccharide Induced Inflammation and Apoptosis of Caco-2 Cells

Shaoyaotang Restores Th17/Treg Cell Balance by Regulating Glucose Metabolism Reprogramming in Treatment of Ulcerative Colitis

Related Author

ZHAO Feiyan

ZHU Wenting

TAN Lin

LUO Wenyu

WANG Xiaoya

BI Xin

WANG Xuecheng

WU Zhenfeng

Related Institution

Jiangxi University of Chinese Medicine

College of Food Science and Technology，Nanchang University

School of Life Sciences，Beijing University of Chinese Medicine

School of Basic Medical Sciences， Chengdu University of Traditional Chinese Medicine（TCM）

School of Basic Medical Sciences， Guangzhou University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰