Determination of Six Active Flavonoids in  Based on HPLC-DAD

CAO Guang-shang; YANG Pei-min; LI Fang; LI Jun

doi:10.13422/j.cnki.syfjx.2014210052

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Determination of Six Active Flavonoids in Based on HPLC-DAD

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- Determination of Six Active Flavonoids in Based on HPLC-DAD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 52-55(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210052
  CLC： R284.1
- Published：2014
- 稿件说明：
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CAO Guang-shang, YANG Pei-min, LI Fang, et al. Determination of Six Active Flavonoids in Based on HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 52-55.
DOI：

CAO Guang-shang, YANG Pei-min, LI Fang, et al. Determination of Six Active Flavonoids in Based on HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 52-55. DOI： 10.13422/j.cnki.syfjx.2014210052.

摘要

目的: 建立HPLC-DAD同时测定白花蛇舌草中6个活性黄酮成分(槲皮素-3-O-桑布双糖苷、芦丁、槲皮素-3-O-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-吡喃葡萄糖苷、槲皮素、山柰酚)含量的方法。方法: 采用Kromasil-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.05% 磷酸梯度洗脱

检测波长254 nm

体积流量1 mL·min-1

柱温30 ℃。结果: 6种活性黄酮成分线性、精密度、稳定性、重复性均符合方法学相关要求

加样回收率为 99.40% ～99.70%。应用所建方法测定了不同产地白花蛇舌草中 6 个活性黄酮成分的量。结论: 所建方法简单、可行、准确

可用于白花蛇舌草中活性黄酮成分的质量控制。

Abstract

Objective: To develop a method of high performance liquid chromatography coupled with a photodiode array detector(HPLC-DAD)for the content determination of six active flavonoids (quercetin-3-O-sambubioside

rutin and quercetin-3-O-β-D-glucopyranside

kaempferol-3-O-β-D-glucopyranside

quercetin

kaempferol)in Oldenlandia diffusa. Method: The analyses were performed on a Diamonsil-C18 column (4.6 mm×250 mm

5 μm) eluted with acetonitrile and 0.05% phosphoric acid in gradient mode. The flow rate was 1 mL·min-1

the detection wavelength was 254 nm

the column temperature was set at 30 ℃. Result: Six flavonoids have good linear relationship

precision

stability

and repeatability according to the requirements of the methodology determination. The recoveries were 99.40% -99.70%. Six flavonoids in O. diffusa from different origins were determined by HPLC-DAD. Conclusion: The method is simple

accurate

replicable

and can be used for the quality control of flavonoids in olden landia diffusa.

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