Isolation and Determination of Verbascoside from

GAO Wei; LIU Bu-ming; CHEN Ming-sheng; HUANG Yan

doi:10.13422/j.cnki.syfjx.2014210060

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Isolation and Determination of Verbascoside from

更新时间：2024-01-04

- Isolation and Determination of Verbascoside from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 60-62(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210060
  CLC： R284.1
- Published：2014
- 稿件说明：
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GAO Wei, LIU Bu-ming, CHEN Ming-sheng, et al. Isolation and Determination of Verbascoside from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 60-62.
DOI：

GAO Wei, LIU Bu-ming, CHEN Ming-sheng, et al. Isolation and Determination of Verbascoside from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 60-62. DOI： 10.13422/j.cnki.syfjx.2014210060.

摘要

目的: 对尖尾枫中毛蕊花糖苷进行提取分离鉴定及含量测定。方法: 采用95% 乙醇对尖尾枫药材进行提取

利用柱色谱和制备型高效液相色谱法对提取物进行分离纯化

通过理化性质鉴别和波谱数据鉴定其结构;采用高效液相色谱法测定尖尾枫中毛蕊花糖苷的含量

ECOSIL ODS-EXTEND色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.2% 磷酸水溶液梯度洗脱

流速1.0 mL·min-1

检测波长334 nm

柱温25 ℃

进样量10 μL。结果毛蕊花糖苷在0.051 9~0.829 6 μg线性关系良好(r=0.999 7)

平均加样回收率为99.13%

RSD 1.80%。结论: 毛蕊花糖苷为首次从该植物中分离得到;该测定方法简便可行

重复性好

为尖尾枫药材的质量控制提供了可靠的检测方法。

Abstract

Objective: To study the isolation and determination of verbascoside from Callicarpa longissima. Method: Verbascoside was extracted by 95% ethanol

isolated and purified by column chromatography and PHPLC from the stem and branch of C. longissima. A HPLC method was set up to determine the content of verbascoside in C. longissimae

using ECOSIL ODS-EXTEND(4.6 mm×250 mm

5 μm) column

and acetonitrile -0.2% phosphoric acid water solution as the mobil1-9e phase

the UV detection wavelength was at 334 nm

with the flow rate of 1.0 mL·min-1 at room temperature. Result: A good linear was obtained in the range of 0.051 9-0.829 6 μg and the average recovery was 99.13% (RSD 1.80%). Conclusion: Verbascoside was isolated from C. longissima for the first time.The method can provide useful references to quality control of C. longissima.

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Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

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