Objective: To establish method of analyze volatile constituents from Artemisiae Argyi Folium by headspace solid-phase microextraction with gas chromatography-mass spectrometry (HS-SPME-GC-MS) technique

and analysis different factors' results on extraction effect. Method: After single factor test

the sum of the peak area of the volatile constituents was as index to optimum extraction conditions

while consider pair wise interactions. Result: The optimum HS-SPME parameters were selected as:1.0 g sample

80 ℃ extracted for 40 minutes. The volatiles were extracted using a 100 μm polydimethylsiloxane (PDMS) fiber

the desorption time was 3 minutes at 250 ℃ and adjustable depth gauge was #2.Analyzing the sample with the optimum methods

a total of 84 chromatographic peaks were resolved

and 66 constituents were identified

which accounted for 94.46% of the total relative contents. Conclusion: Extraction temperature is the main factor

followed by the interaction between the extraction temperature and sample quality

then the extraction time

and finally the quality of the sample. Other factors have a significant impact on the extraction effect in addition to the sample quality (P<0.05). It provides the basis for development and utilization of the Artemisiae Argyi Folium.