Objective: A rapid and sensitive method for HPLC was established to simultaneouly determine chlorogenic acid

forsythin

baicalin

wogonin and apigenin. Method: Chromatographic separation was carried out on Agilent Eclipse XDB-C18 column(4.6 mm×250 mm

5 μm) under gradient elution(0-0.01 min

A phase is 25%;0.01-10 min

A phase is 25% -53%;10-16 min

A phase is 53% -78%) with methanol (A phase)-water/0.2% phosphate(B phase) as mobile phase at a flow rate of 1.00 mL·min-1 to elute at 25 ℃. The injection volume was 20 μL and the detection was adopted UV detector at 278 nm. Result: These five components were seperated basically within 25 minutes with good linearity.The regression equations were Y=2 350.4X-137.27

r=0.999 6(for chlorogenic acid)

Y=1 161.7X+18.474

r=0.999 6(for forsythin)

Y=3 204.2X-423.45

r=0.998 8(for baicalin)

Y=4 792.7X-93.15

r=0.998 5(for wogonin)

Y=1 434.1X-8.413 2

r=0.999 3(for apigenin).The average recoveries were:chlorogenic acid 99.47% (RSD 0.8%)

forsythin 98.26% (RSD 0.9%)

baicalin 100.4% (RSD 1.9%)

wogonin 99.29% (RSD 1.1%)

apigenin 98.79% (RSD 1.1%). Conclusion: The HPLC method has good accuracy

reliability

and repeatbility.It is suitable for compound Honeysuckle granules and traditonal Chinese medicines containing the same ingredients to improve their quality control.