Chemical Constituents and Antitumor Activity of Fatty Oil from  Seeds

QIU Yan; CHEN Yong; CHEN Jian-wei; WANG Yu; XU Sha-sha; QIU Hai-long; LI Xiang

doi:10.13422/j.cnki.syfjx.2014210109

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Chemical Constituents and Antitumor Activity of Fatty Oil from Seeds

更新时间：2024-01-04

- Chemical Constituents and Antitumor Activity of Fatty Oil from Seeds
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 109-111(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210109
  CLC：
- Published：2014
- 稿件说明：
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QIU Yan, CHEN Yong, CHEN Jian-wei, et al. Chemical Constituents and Antitumor Activity of Fatty Oil from Seeds[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 109-111.
DOI：

QIU Yan, CHEN Yong, CHEN Jian-wei, et al. Chemical Constituents and Antitumor Activity of Fatty Oil from Seeds[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 109-111. DOI： 10.13422/j.cnki.syfjx.2014210109.

摘要

目的: 研究番荔枝子脂肪油化学成分及体外抑制肿瘤细胞增殖活性。方法: 对番荔枝子脂肪油进行甲酯化后

应用气相色谱(GC)分析与混合对照品对照

分析其主要化学成分及采用峰面积归一化法测定其相对含量。选取人肝癌细胞(SMMC-7721和HepG2)

人宫颈癌细胞(Hela)

人肺癌细胞(A-549)

人乳腺癌细胞(MCF-7)

用MTT法研究番荔枝子脂肪油的体外抑制肿瘤细胞增殖活性。结果: 番荔枝子脂肪油部位脂肪酸的相对质量分数为80.9%

其中不饱和脂肪酸的相对质量分数达51.1%;番荔枝子脂肪油对上述5种人肿瘤细胞抑制作用半数抑制浓度(IC50)分别为10.4

0.57

30.3

44.3

6.7 mg·L-1。结论: 番荔枝子脂肪油中主要含有油酸、亚油酸、棕榈酸和硬脂酸

其对人肝癌HepG2细胞有选择性抑制其增殖作用。

Abstract

Objective: To investigate the components and antitumor activity against human tumor cell lines of fatty oil of Annona squamosa seeds. Method: The samples were derivated by methyl esterification

the components were identified by gas chromatography (GC) analysis and comparison of references

and the relative amount of these components were determined by peak area normalization method. In vitro anti-proliferative activity of the fatty oil against human liver cancer (SMMC-7721 and HepG2)

human uterine cervix cancer (Hela)

human lung cancer (A549) and human breast cancer (MCF-7) cell lines were tested by MTT assay. Result: The major chemical constituents of the fatty oil were fatty acid (80.9%)

and the content of unsaturated fatty acid were 51.1%. The IC50 of the fatty oil against the 5 human cancer cell lines tested above was 10.4

0.57

30.3

44.3

6.7 mg·L-1 respectively. Conclusion: The main components of the fatty oil of A. squamosa seeds were methyl palmitate

methyl stearate

methyl oleate and methyl linoleicacid. They showed high selective inhibitation towards the proliferation of HepG2 cell line.

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