Objective: To establish HPLC fingerprint for comparative studying on chemical substances difference between stems and leaves of Uncariae Ramulus Cum Uncis

in order to lay scientific basis for follow-up studies of Uncariae Ramulus Cum Uncis leaves. Method: Similarity of HPLC fingerprint was calculated by 2004A version of "traditional medicine fingerprint similarity software evaluation system" for 10 batches of stems and 10 batches of leaves in Uncariae Ramulus Cum Uncis.Taking rhynchophylline and isorhynchophylline as index components

HPLC analysis was performed on a Kromasil column(4.6 mm×250 mm

5 μm) with gradient eluting system consisted of methanol(A)-0.02% ammonia(B)(0-45 min

30% -60% A;45-75 min

60% -64% A;75-90 min

64% -70% A;90-95 min

70% -70% A)

flow rate was 1.0 mL·min-1

column temperature was maintained at 30 ℃ and detection wavelength was set at 245 nm

injection volume of 10 μL. Result: Sixteen common peaks were obtained in 10 batches of Uncariae Ramulus Cum Uncis stems with similarity higher than 0.87 in each batches of samples;sixteen common peaks were obtained in 10 batches of Uncariae Ramulus Cum Uncis leaves with similarity higher than 0.80 in each batches of samples. Conclusion: The content of isorhynchophylline in leaves is higher than that in leaves

but the content of rhynchophylline have little difference.This established fingerprint conditions can be used as initial comparative studies on main chemical components of Uncariae Ramulus Cum Uncis stems and leaves

it provide a reference for medicinal research of Uncariae Ramulus Cum Uncis leaves.