Long-term Application of Diazepam Lead to Body Tolerance in Mice and Inhibition of Sedative Chinese Medicine Pairs on Its Role

DING Li; PAN Yi-hong; YOU Qiu-yun; WANG Ping

doi:10.13422/j.cnki.syfjx.2014210140

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Long-term Application of Diazepam Lead to Body Tolerance in Mice and Inhibition of Sedative Chinese Medicine Pairs on Its Role

更新时间：2024-01-04

- Long-term Application of Diazepam Lead to Body Tolerance in Mice and Inhibition of Sedative Chinese Medicine Pairs on Its Role
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 140-143(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210140
  CLC： R285.5
- Published：2014
- 稿件说明：
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DING Li, PAN Yi-hong, YOU Qiu-yun, et al. Long-term Application of Diazepam Lead to Body Tolerance in Mice and Inhibition of Sedative Chinese Medicine Pairs on Its Role[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 140-143.
DOI：

DING Li, PAN Yi-hong, YOU Qiu-yun, et al. Long-term Application of Diazepam Lead to Body Tolerance in Mice and Inhibition of Sedative Chinese Medicine Pairs on Its Role[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 140-143. DOI： 10.13422/j.cnki.syfjx.2014210140.

摘要

目的: 观察长期应用地西泮小鼠对药物的耐受性及不同安神药对对其的抑制作用

并初步探讨其机制。方法: 将小鼠分为空白组、模型组、法半夏-秫米组(3.25 g·kg-1·d-1)、磁石-朱砂组(0.33 g·kg-1·d-1)、黄连-阿胶组(2.73 g·kg-1·d-1)、黄连-肉桂组(2.15 g·kg-1·d-1)、栀子-淡豆豉组(1.69 g·kg-1·d-1)

每天灌胃(ig) 1次

连续60 d。给药期间

除空白组外

各组隔2日于ig给药30 min后每3天1次ip地西泮注射液25 mg·kg-1

造成地西泮耐受。持续监测小鼠睡眠潜伏期

测定自主活动次数和时间

并用荧光定量PCR测定小鼠全脑γ-氨基丁酸受体(GABAA-R)mRNA、谷氨酸脱羧酶65(GAD65)mRNA的表达。结果: 用药期间

模型组睡眠潜伏期逐渐延长

黄连-肉桂组、黄连-阿胶组睡眠潜伏期变化不大;用药2个月后

与模型组比较

黄连-肉桂组、黄连-阿胶组睡眠潜伏期明显缩短(P<0.01)

黄连-肉桂组、黄连-阿胶组、法半夏-秫米组小鼠自主活动次数、活动时间显著性减少(P<0.05

P<0.01)

黄连-肉桂组、黄连-阿胶组、半夏-秫米组、磁石-朱砂组和栀子-淡豆豉组小鼠脑组织GABAA-R mRNA

GAD65 mRNA表达显著性上调(P<0.05

P<0.01)。结论: 黄连-阿胶、黄连-肉桂和法半夏-秫米具有抑制长期单独应用地西泮而致药效下降机体耐受的作用

其机制可能与上调脑内GABAA-R和GAD65有关。

Abstract

Objective: To observe the long-term application of diazepam on drug tolerance in mice and different sedative medicine for its inhibitory effect

and discussed its mechanism. Method: Mice were divided into blank control group

model control group

Pinelliae Rhizoma-Husked Sorghum group(3.25 g·kg-1·d-1)

Magnetitum-Cinnabaris group(0.33 g·kg-1·d-1)

Coptidis Rhizoma-Asini Corii Colla group(2.73 g·kg-1·d-1)

Coptidis Rhizoma-Cinnamomi Cortex group(2.15 g·kg-1·d-1) and Gardeniae Fructus-Sojae Semen Praeparatum group(1.69 g·kg-1·d-1)

orally once a day for 60 days.During the 60 days

except the blank control group

each group mice were injected with diazepam in 30 minutes after taking drugs(25 mg·kg-1·3 d-1).Continuously monitor the mice sleep latency

determination of autonomic activity frequency and time

using fluorescence quantitative PCR determination of whole brain gamma-aminobutyric acid receptors(GABAA-R) mRNA in mice

glutamic acid decarboxy lase 65(GAD65) mRNA expression. Result: During the medication

sleep latency period of diazepam group extended gradually

Coptidis Rhizoma-Asini Corii Colla group and Coptidis Rhizoma-Cinnamomi Cortex group changed little;Medication after 2 months

compared with diazepam group

sleep latency period of Coptidis Rhizoma-Asini Corii Colla group and Coptidis Rhizoma-Cinnamomi Cortex group was obviously shortened(P<0.01)

and mice autonomic activity times and activity time of Coptidis Rhizoma-Asini Corii Colla group

Coptidis Rhizoma-Cinnamomi Cortex group and Pinelliae Rhizoma-Husked Sorghum group significantly reduced (P<0.05

P<0.01)

and Coptidis Rhizoma-Asinicorii Colla group

Coptidis Rhizoma-Cinnamomi Cortex group

Pinelliae Rhizoma-Husked Sorghum group

magnetitum-cinnabaris group and gardeniaefructus-sojaesemenpraeparatum group mice brain GABAA-R mRNA and GAD65 mRNA expression significantly higher (P<0.05

P<0.01). Conclusion: Coptidis Rhizoma-Asini Corii Colla

Coptidis Rhizoma-Cinnamomi Cortex and Pinelliae Rhizoma-Husked Sorghum can alleviate body tolerance in mice because of the long-term application of diazepam alone

the mechanism may be related to increase GABAA-R and GAD65 in the brain.

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