Objective: Observe the protective effects of Bushen Houxue fang(BSHXF) on 6-hydroxydopamine (6-OHDA) type of Parkinson's disease in substantia nigra of injection of 6-OHD rat dopaminergic neurons. Method: Substantia nigra once injection of 4 μL 6-OHDA to model the Parkinson's disease rat

and divide those models into 6 different groups: normal control (0.9% NaCl)model (0.9% NaCl)

madopar comparison (20 mg·kg-1 madopar)

high (15 g·kg-1 BSHXF)

mid (10 g·kg-1) and low (5 g·kg-1) dosage of BSHXF. Each group intragastric administration one time every day for 15 days. Using ethology detection

immunohistochemistry to determine substantia nigra tyrosine hydroxylase

SOD

MDA and GSH-Px

then observe the protective effects of Bushen Houxue fang on DA neurons. Result: ①Group model rotates clockwise (11.27±1.68) lap/min

there is significant difference (P<0.05) among High dosage of BSHXF group (6.35± 1.49 lap/min

madobar group (5.76±1.23)lap/min and model group. ②Madobar and high dosage group compare to model group have increasing activity of substantia nigra (P<0.05)

as well as GSH-Px (P<0.01). Meanwhile

high dosage of BSHXF and madobar have similar increase in SOD

GSH-Px and decrease in MDA. ③TH immunohistochemistry result shows BSHXF high dosage and madobar group rat substantia nigra TH have large number of positive neuron

large soma size

significant outgrowth

the comparison of the two groups shows no statistical difference. PD model group has significant decrease in the number of neuron (P<0.01)

even disappearing

neuron soma atrophy

little outgrowth. Conclusion: There are protective effects of BSHXF on 6-OHDA type of Parkinson's disease in substantia nigra of rat dopaminergic neurons

its effect mechanism could achieved through anti-oxidative stress and increase level of tyrosine hydroxylase in substantia nigra.