Objective: To observe the immune promotion and anti-fatigue of effect of Exocarpium citri polysaccharide in mice. Method: KM mice were randomly divided into control group

model group

Zhenqi Fuzheng granule group (10 g·kg-1)

E. citri polysaccharide low dose group (23 mg·kg-1)

middle dose group (46 mg·kg-1)

high dose group (92 mg·kg-1).The groups were gavaged(ig)with drug daily for 30 consecutive days

at dose of 10 mL·kg-1. On 26th day

the delayed-type hypersensitivity animal model in mice was established by sensation of 2

4-dinitro fluoro benzene(DNFB)

the control group was treated with the solvent without DNFB

the weight of right and left ears was measured at 24 h after challenged.Since the third week

dexamethasone was injected to all the groups except for control group every two days to establish immunosuppressed mice model

40 mg·kg-1 dose

five times.Observed spleen index

thymus index at 1 h after last objection. Using swimming and anti hypoxia experiment to observe the anti fatigue effect. Result: The effect of Zhenqi Fuzheng granules group

the polysaccharide groups at 46

92 mg·kg-1 could enhance the delayed type hypersensitivity(P<0.01

P<0.05)

the difference of mouse ear swelling was (6.57± 1.67)

(8.29±2.41)mg

respectively.Zhenqi Fuzheng granule group

polysaccharide group at 92 mg·kg-1 could significantly increase the immune suppression of thymus and spleen index in mice(P<0.01

P<0.05). Zhenqi Fuzheng granules group

polysaccharide groups at 23

46

92 mg·kg-1 could significantly prolong the swimming time

swimming time was (286±46)

(188± 34)

(227±28)

(291±48)s. Zhenqi Fuzheng granule group

polysaccharide groups at 23

46

92 mg·kg-1 could significantly prolong the hypoxia tolerance time of mice(P<0.01

P <0.05). The effect of enhancement of immune function and anti fatigue action showed a manner of dose depent. Conclusion: E. citri polysaccharide can enhance immunity

and have anti-fatigue effect. Polysaccharide is the effective chemical fraction of E. citri.