Preparation and Antibacterial Antioxidant Activity of Phillyrin, Forsythiaside A on -butanol Part Extract from Qingqiao of

ZHANG Ying-juan; HAN Yan-xia; LIANG Li-peng; SHANG Cai-ling; XUE Hui-qing

doi:10.13422/j.cnki.syfjx.2014210192

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Preparation and Antibacterial Antioxidant Activity of Phillyrin, Forsythiaside A on -butanol Part Extract from Qingqiao of

更新时间：2024-01-04

- Preparation and Antibacterial Antioxidant Activity of Phillyrin, Forsythiaside A on -butanol Part Extract from Qingqiao of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 21, Pages: 192-196(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014210192
  CLC： R285.5
- Published：2014
- 稿件说明：
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ZHANG Ying-juan, HAN Yan-xia, LIANG Li-peng, et al. Preparation and Antibacterial Antioxidant Activity of Phillyrin, Forsythiaside A on -butanol Part Extract from Qingqiao of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 192-196.
DOI：

ZHANG Ying-juan, HAN Yan-xia, LIANG Li-peng, et al. Preparation and Antibacterial Antioxidant Activity of Phillyrin, Forsythiaside A on -butanol Part Extract from Qingqiao of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(21): 192-196. DOI： 10.13422/j.cnki.syfjx.2014210192.

摘要

目的: 从青翘正丁醇部位制备连翘苷及连翘酯苷A单体化合物

并对其抑菌活性及清除DPPH自由基的抗氧化能力进行研究。方法: 采用硅胶柱层析、重结晶等技术对青翘正丁醇部位连翘苷及连翘酯苷A进行分离纯化;采用K-B纸片扩散法测定连翘苷、连翘酯苷A对大肠埃希菌、金黄色葡萄球菌、肺炎链球菌的抑菌效果;DPPH法测定连翘苷、连翘酯苷A清除DPPH自由基的抗氧化能力。结果: 连翘酯苷A对大肠埃希菌、肺炎链球菌及金黄色葡萄球菌抑菌圈的直径分别为12.35

9.58

12.29 mm

连翘酯苷A的抑菌活性高于青翘正丁醇部位总浸膏的抑菌活性

低于庆大霉素的抑菌活性

而连翘苷无抑菌活性

连翘酯苷A对3种细菌的抑制强弱为:大肠埃希菌﹥金黄色葡萄球菌﹥肺炎链球菌;连翘酯苷A清除DPPH自由基的能力大于连翘苷。结论: 连翘酯苷A可能是青翘抑菌活性及抗氧化活性的主要成分。

Abstract

Objective: To preparate the phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao of Forsythia suspensa

and to explore their antibacterial activity and free DPPH radical scavenging capacity. Method: The phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao were isolated and purified by means of silica gel column chromatography and recrystallization

etc. The antibacterial activity of phillyrin and forsythiaside A were tested against Escherichia coli

Staphylococcus aureus and Streptococcus pneumoniae by using K-B paper disk method. DPPH assay was employed to assess the antioxidant activity of the phillyrin and forsythiaside A. Result: The diameter of inhibition zone forming by forsythiaside A against E. coli

S. pneumoniae and S. aureus were 12.35

9.58

12.29 mm

respectively. In vitro antibacterial tests revealed that forsythiaside A exhibited better effect than n-butanol fraction of Qingqiao

but not good as gentamicin

and phillyrin didn't show any activity. Meanwhile

forsythiaside A was more potent against E. coli than other two strains. Morever

DPPH experimental results showed that forsythiaside A had higher free DPPH radical scavenging capacity than the phillyrin. Conclusion: Forsythiaside A showed a higher antibacterial activity and antioxidant activity in vitro than phillyrin

and it may be the main active components of Qingqiao.

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