LUO Yun, XIONG Zhi-wei, LIANG Xin-li, et al. Optimization of Matrix Formulation and Content Determination of Paridis Rhizoma Total Saponins Cataplasms[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 5-9.
DOI:
LUO Yun, XIONG Zhi-wei, LIANG Xin-li, et al. Optimization of Matrix Formulation and Content Determination of Paridis Rhizoma Total Saponins Cataplasms[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 5-9. DOI: 10.13422/j.cnki.syfjx.2014220005.
Optimization of Matrix Formulation and Content Determination of Paridis Rhizoma Total Saponins Cataplasms
Objective:To optimize matrix formulation of Paridis Rhizoma total saponins cataplasms and establish its determination. Method: With ductility
fabric penetration
uniformity
shaped type
skin following nature
adhesion and repeated exposing paste as comprehensive evaluation indicators
on the basis of preliminary experiments
taking dosages of gelatin
sodium polyacrylate
glycerine and polyvinylpyrrolidone-K30(PVP-K30) as factors
matrix formulation of this preparation was optimized by uniform design.HPLC was adopted to determine contents of polyphyllin Ⅱ and polyphyllin Ⅶ with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-40 min
30%-60%A;40-50 min
60%-30%A;50-55 min
30%A)
flow rate of 1.0 mL · min-1 and detection wavelength at 203 nm. Result: Optimum matrix formulation was gelatin-sodium polyacrylate-PVP-K30-glycerine-glycinate aluminum-azone-total saponins from Paridis Rhizoma(2.0: 4.0: 1.25: 15: 0.16: 1.2: 4);polyphyllin Ⅱ and polyphyllin Ⅶ had good liner relationship in the ranges of 0.055-5.5 μg and 0.086 8-8.68 μg
respectively;contents of them in this cataplasms were 1.06
3.79 mg · g-1
respectively. Conclusion: Prepared cataplasms have great drug loading capacity
good adhesion and appearance.Established HPLC method is convenient
accurate and reliable with good reproducibility
which can be suitable for quality evaluation of this preparation.