Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin

WANG Shao-ping; ZENG Yi-wang; CHEN Yan-rui; LIU Guo-fei; DAI Long

doi:10.13422/j.cnki.syfjx.2014220009

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Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin

更新时间：2024-01-04

- Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 9-11(2014)
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- DOI：10.13422/j.cnki.syfjx.2014220009
  CLC： R283.6;R284.1;R284.2;R282.74
- Published：2014
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WANG Shao-ping, ZENG Yi-wang, CHEN Yan-rui, et al. Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 9-11.
DOI：

WANG Shao-ping, ZENG Yi-wang, CHEN Yan-rui, et al. Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 9-11. DOI： 10.13422/j.cnki.syfjx.2014220009.

摘要

目的: 考察DA-201C型大孔树脂对土鳖虫酶解液的脱盐纯化效果.方法: 采用DA-201C型树脂对土鳖虫酶解液进行脱盐试验

通过单因素试验考察上样液pH和质量浓度、洗脱剂种类和用量对土鳖虫小肽的影响.通过folin-酚法测定活性成分小肽含量

检测波长650 nm

利用电导率测定除盐率.结果: 最佳除盐工艺为上样液与树脂柱体积比1:1

上样液质量浓度10 mg ·L-1

上样液pH 7.0

吸附流速1.5 BV ·h-1

加水3 BV洗除杂

加75%乙醇3 BV洗脱.除盐率99.1%

小肽回收率86.05%.结论: 采用DA-201C型大孔树脂对土鳖虫酶解液进行纯化除盐处理的方法简便、易行.

Abstract

Objective:To investigate desalination purification effect of Eupolyphaga enzymatic hydrolysate by DA-201C macroporous resin. Method: Desalination test of Eupolyphaga enzymatic hydrolysate was adopted by DA-201C macroporous resin

effects of pH and concentration of sample solution

type and amount of eluent on Eupolyphaga small peptides were investigated by single factor tests.Folin method was employed to determine the content of small peptides with detection wavelength at 650 nm. Result: Optimum desalination process was as follows:resin-sample solution of 1: 1

the concentration of sample solution for 10 mg · L-1

pH of 7.0

adsorption rate of 1.5 BV · h-1

washed impurity with 3 BV of water and eluted by 3 BV of 75% ethanol;desalination rate was 99.1% with small peptides recovery of 86.05%. Conclusion: This optimized desalination process is simple and feasible

it can achieve desired purpose of Eupolyphaga enzymatic hydrolysate.

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