Optimization of Extraction Technology of Total Saponins from  Roots by Central Composite Design-response Surface Methodology

TENG Kun; RUAN Hong-sheng; WU Zi-jing; ZHANG Li-qiu; ZHAO Rui

doi:10.13422/j.cnki.syfjx.2014220026

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Optimization of Extraction Technology of Total Saponins from Roots by Central Composite Design-response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Technology of Total Saponins from Roots by Central Composite Design-response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 26-28(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014220026
  CLC： R283.6;R284.1;R284.2;R282.71
- Published：2014
- 稿件说明：
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TENG Kun, RUAN Hong-sheng, WU Zi-jing, et al. Optimization of Extraction Technology of Total Saponins from Roots by Central Composite Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 26-28.
DOI：

TENG Kun, RUAN Hong-sheng, WU Zi-jing, et al. Optimization of Extraction Technology of Total Saponins from Roots by Central Composite Design-response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 26-28. DOI： 10.13422/j.cnki.syfjx.2014220026.

摘要

目的: 优选藤梨根总皂苷的提取工艺

为该药用部分的开发提供参考.方法: 以总皂苷提取量为指标

采用星点设计-响应面法考察提取时间、乙醇体积分数和料液比对藤梨根总皂苷提取工艺的影响.利用UV测定总皂苷含量

检测波长547 nm.结果: 最佳工艺条件为提取时间73 min

乙醇体积分数65%

料液比1:13;藤梨根总皂苷提取量1.67 mg ·g-1

与预测值1.69 mg ·g-1的偏差1.18%.结论: 该提取工艺稳定可行

适用于藤梨根总皂苷的提取.

Abstract

Objective:To optimize extraction technology of total saponins from Actinidia arguta roots and provide a reference for development of this medicinal part. Method: With extraction time

ethanol concentration and ratio of solid to liquid as independent variables

extracting amount of total saponins as dependent variable

extraction technology of total saponins from Actinidia arguta roots was optimized by central composite design-response surface methodology.UV was employed to determine the content of total saponins with detection wavelength at 547 nm. Result: Optimum technological parameters were as follows:extraction time of 73 min

ethanol concentration of 65%

solid-liquid of 1: 13;extracting amount of total saponins was 1.67 mg · g-1

which was coincided with the model predictive value of 1.69 mg · g-1. Conclusion: This optimized extraction technology is simple

reliable and suitable for extracting total saponins from A. arguta roots.

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