SUN Yao, LI Chun-feng, LIN Wei-xin, et al. Orthogonal Design for Optimizing Purification Process of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 29-32.
DOI:
SUN Yao, LI Chun-feng, LIN Wei-xin, et al. Orthogonal Design for Optimizing Purification Process of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 29-32. DOI: 10.13422/j.cnki.syfjx.2014220029.
Orthogonal Design for Optimizing Purification Process of Total Flavonoids from
Objective:To optimize purification process of total flavonoids from Bauhinia championii by D101 macroporous resin and provide a reference for preparations development of these components. Method: With purity and recovery of total flavonoids as indexes
based on single factor tests
orthogonal design was used to optimize purification process by taking eluent concentration
elution rate
pH and concentration of sample solution as factors.UV was employed to determine the content of baicalin with detection wavelength at 278.5 nm. Result: Optimal purification process was as following:the concentration of sample solution 15.5 g · L-1
pH of sample solution 2-3
the maximum sample volume of 16.85 mg · g-1
sampling rate of 0.125 BV · h-1
diameter and height ratio of 1: 6
eluted by 4 BV of 60% ethanol with elution speed of 1 BV · h-1.Under this process
purity and recovery were 48.95% and 96.08%
respectively. Conclusion: This optimized purification process is stable
feasible and suitable for purifying total flavonoids from B. championii
which can effectively improve its purity and recovery.
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