Objective:The aim of this study was to establish a HPLC method for simultaneous determination of the contents of plantamajoside

polydatin

specnuezhenide and resveratrol in Huzhentongfeng capsules. Method: HPLC was used to determine the contents. The analytical column was Agilent SB-C18 column (4.6 mm×250 mm

5 μm). The liquid phase was composed of acetonitrile and 0.1% formic acid with gradient elution. The detection wavelength was set at 280 nm

the flow rate was 1.0 mL · min-1 and the column temperature was maintained at 30 ℃. Result: The mass of plantamajoside

polydatin

specnuezhenide and resveratrol with peak areas were within the concentration ranges of 0.099 6-1.992 μg(r=0.999 9)

0.123-2.46 μg(r=0.999 9)

0.145-2.90 μg(r=0.999 9)

0.025-0.50 μg(r=0.999 8)

and the average recoveries were 99.27%

99.43%

98.83%

98.07%

respectively. Conclusion: The method is rapid and simple

highly reproducible and specific so that it could be used as one of the quality control methods of Huzhentongfeng capsules.