JIANG Mei, TAN Li-rong, HUANG Xiao-jie, et al. Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 53-56.
DOI:
JIANG Mei, TAN Li-rong, HUANG Xiao-jie, et al. Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 53-56. DOI: 10.13422/j.cnki.syfjx.2014220053.
Pre-column Derivatives HPLC Fingerprint of Lycii Fructus Polysaccharides
采用HPLC测定枸杞子多糖中单糖的衍生物.采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm
5 μm)
流动相0.1 mol ·L-1磷酸缓冲液(pH 6.7)-乙腈(84.5:15.5)
流速0.8 mL ·min-1
检测波长250 nm
柱温30 ℃.结果: 枸杞子多糖柱前衍生指纹图谱标示出11个共有峰
鉴别了8个共有峰(D-甘露糖
L-鼠李糖
D-葡萄糖醛酸
D-半乳糖醛酸
D-葡萄糖
D-半乳糖
D-木糖
D-阿拉伯糖).10批枸杞子药材指纹图谱相似度>0.99.结论: 该方法简便
分离度高
重复性及稳定性良好
可有效控制枸杞子多糖的质量.
Abstract
Objective:The aim of this study was to establish a pre-column derivation HPLC method for chromatographic fingerprint of Lycii Fructus Polysaccharide. Method: Lycii Fructus sample was hydrolyzed with Trifluoroacetic Acid(TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone(PMP). The monosaccharide composition of Lycii Fructus polysaccharide was carried out by reversed-phase technique on a ZORBAX Eclipse XDB-C18 column with a mobile phase composed of 0.1 mol · L-1 phosphate buffer (pH 6.7) and acetonitrile in the ratio of 84.5: 15.5.The flow rate was set at 0.8 mL · min-1
the wavelength was set at 250 nm
and the column temperature was at 30 ℃. Result: There were 12 peaks from the fingerprint and 8 peaks were D-Man
L-Rha
D-GlcUA
D-GlcN
D-Glc
D-Gal
D-Xyl
D-Ara. The similarity of the 10 batches of Lycium barbarum samples was more than 0.99. Conclusion: The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Lycii Fructus polysaccharide and the method is simple
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