Simultaneous Determination of Four Alkaloids in Wuqi Qufengsan Tablets by HPLC

MAO Kun-jun; PAN Yi-lin; LI Fu-qiang; BAI Gang-gang; LI Xiang; CHEN Jian-wei

doi:10.13422/j.cnki.syfjx.2014220074

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Four Alkaloids in Wuqi Qufengsan Tablets by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Four Alkaloids in Wuqi Qufengsan Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 74-77(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014220074
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
MAO Kun-jun, PAN Yi-lin, LI Fu-qiang, et al. Simultaneous Determination of Four Alkaloids in Wuqi Qufengsan Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 74-77.
DOI：

MAO Kun-jun, PAN Yi-lin, LI Fu-qiang, et al. Simultaneous Determination of Four Alkaloids in Wuqi Qufengsan Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 74-77. DOI： 10.13422/j.cnki.syfjx.2014220074.

摘要

目的: 建立乌七祛风散片(制草乌、三七、断节参、雪上一枝蒿、防风等9味中药组成)中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和次乌头碱的HPLC测定方法.方法: 色谱条件

Hanbon-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相:[乙腈-四氢呋喃(25:15)](A)-0.1 mol ·L-1醋酸铵溶液(每1 000 mL加冰醋酸0.5 mL)(B)

流速1.0 mL ·min-1

柱温30 ℃

检测波长232 nm.结果: 4种生物碱分离良好

各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.999 0)

加样回收率分别为101.10%(RSD 2.1%)

101.09%(RSD 2.2%)

102.04%(RSD 1.6%)

97.83%(RSD 2.7%)

最低检测限分别为3.4

1.7

2.2

0.8 ng.结论: 该HPLC方法稳定可靠、简便易行

可用于同时测定乌七祛风散片中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和次乌头碱含量.

Abstract

Objective:To establish an HPLC method for simultaneous determination of benzoylmesaconine

benzoylaconine

benzoylhypacoitine and hypaconitine in Wuqi Qufengsan tablets. Method: The assay was performed on a Hanbon-C18(4.6 mm×250 mm

5 μm)

mobile phase consisted of acetonitrile-tetrahydrofuran (25: 15

phase A)-0.1 mol · L-1 ammonium acetate(containing 0.5 mL glacial acetic acid per 1 000 mL

phase B) with gradient elution at the flow rate of 1.0 mL · min-1

column temperature was 30 ℃

and the detection wavelength was at 235 nm. Result: Excellent chromatographic separation was achieved for four studied alkaloids with good linearity(r>0.999 0)

the average recoveries were 101.10%(RSD 2.1%)

101.09%(RSD 2.2%)

102.04%(RSD 1.6%)

97.83%(RSD 2.7%). The limits of detection were 3.4

1.7

2.2and 0.8 ng respectively. Conclusion: This method was simple

accurate and reproducible

it can be used to determine the content of benzoylmesaconine

benzoylaconine

benzoylhypacoitine and hypaconitine in Wuqi Qufengsan tablets.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Monoester-alkaloids from Aconiti Kusnezoffii Radix Cocta in Xiaojin Wan

HPLC Characteristics of Aqueous Extract of Zhenwu Decoction and Determination of Markers

Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta

To Analyse the Toxicity of Tongmai-Sini-Tang

Determination of Aconitine,Mesaconitine,Hypaconitine in Radix Aconitilateralis Preparata by IP-LC

Related Author

REN Gui-lin

HAN Li

WANG Xiao-ping

MEI Ming

HE Jing

TANG Yi-xuan

ZHANG Ding-kun

YANG Ming

Related Institution

Guizhou Normal University Guiyang

Institute of Chinese Materia Medica,China Academy of TCM

中国中医研究院中药研究所北京100700

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰