Objective:To establish a method for determining contents of brucine

strychnine and aconitine in Guci pastes leachate by RP-HPLC. Method: Symmetry C18 column (4.6 mm×250 mm

5 μm)

mobile phase was constituted by acetonitrile(A)-water(B)(containing 0.01 mol · L-1 potassium dihydrogen phosphate solution

adjusted pH to 3 by phosphoric acid) for gradient elution(0-22 min

11%A;22-23 min

11%-33%A;23-36 min

33%A;36-37 min

33%-11%A;37-40 min

11%A) at a flow rate of 1.0 mL · min-1

detection wavelength was set at 235 nm for aconitine

260 nm for brucine and strychnine. Result: Linear ranges of brucine

strychnine and aconitine were 0.026 4-0.264

0.039 6-0.396

0.016-0.160 μg;their average recoveries were 98.44%

98.63% and 99.08% with RSD of 1.84%

1.66% and 0.97%;their average concentrations in samples were 1.29

1.92

0.79 mg · L-1

respectively. Conclusion: This method is simple

accurate and reproducible

it can be provide experimental basis for quality control of Guci pastes.