Alkaloids Constituents of  Seed and Their Cytotoxic Activity

YAO Wen; SUN Pei-huan; XU Xiao-min; LI Ya-juan; YUAN Cheng-shan; WANG Zhen-hua

doi:10.13422/j.cnki.syfjx.2014220095

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Alkaloids Constituents of Seed and Their Cytotoxic Activity

更新时间：2024-01-04

- Alkaloids Constituents of Seed and Their Cytotoxic Activity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 95-99(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014220095
  CLC： R284.1;R284.2
- Published：2014
- 稿件说明：
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YAO Wen, SUN Pei-huan, XU Xiao-min, et al. Alkaloids Constituents of Seed and Their Cytotoxic Activity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 95-99.
DOI：

YAO Wen, SUN Pei-huan, XU Xiao-min, et al. Alkaloids Constituents of Seed and Their Cytotoxic Activity[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 95-99. DOI： 10.13422/j.cnki.syfjx.2014220095.

摘要

目的: 研究苦豆子种子的化学成分并对其抗肿瘤活性进行测试.方法: 采用常规的硅胶柱层析(CC)

葡聚糖(Sephadex LH-20)柱层析

和重结晶等实验手段对其进行了分离纯化

利用波谱技术(1H-NMR

13C-NMR)及与文献数据对照

对分离的化合物进行了结构鉴定;并采用 MTT 测定部分生物碱单体化合物体外对C6肿瘤细胞的细胞毒活性.结果: 共分离出7个生物碱类化合物

分别鉴定为:苦参碱(1)

氧化苦参碱(2)

槐果碱(3)

9α-羟基槐果碱(4)

氧化槐果碱(5)

9α-羟基槐胺碱(6)

(-)-13

14-去氢槐定碱(7)

1个非生物碱化合物3-吲哚甲醛(8).结论: 化合物8为首次从该属植物中分离得到.细胞毒活性测试结果表明

化合物1~3和6对鼠脑胶质瘤C6细胞显示了较强的生长抑制活性.

Abstract

Objective:To investigate the chemical constituents in seed of Sophora alopecuroides and to determine their antitumor activities in vitro. Method: Compounds were isolated and purified by means of silica gel and Sephadex LH-20 gel columm chromatography

and recrystallization etc. Their structures were elucidated using spectroscopic analysis including 1H-NMR

13C-NMR and spectral data. The cytotoxic activities of the alkaloids compounds on C6 cell in vitro were evaluated by MTT method. Result: Seven compounds were isolated from the ethanol extract of the seed of S. alopecuroides. Their structrues were identified as (+)-matrine(1)

(+)-oxymatrine(2)

(-)-sophocarpine(3)

(-)-9α-hydroxysophocarpine(4)

(+)-oxysophocarpine(5)

(-)-9α-hydroxysophoramine(6)

(-)-13

14-dehydrosophoridine(7)

and a non-alkaloid named indolyl-3-carbaldehyde(8). Conclusion: The compond 8 was isolated from the genus sophora for the first time. Compounds 1-3 and 5 showed significant inhibitory activity towards C6 glioma cells.

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Related Institution

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