Simultaneous Determination of Three Components in  by HPLC and Comparison Among Different Extraction Method

GUAN Ying-li; HE Xiao-yan; YAO Hui-min

doi:10.13422/j.cnki.syfjx.2014220100

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Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method

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- Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 100-103(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014220100
  CLC： R284.1
- Published：2014
- 稿件说明：
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GUAN Ying-li, HE Xiao-yan, YAO Hui-min. Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 100-103.
DOI：

GUAN Ying-li, HE Xiao-yan, YAO Hui-min. Simultaneous Determination of Three Components in by HPLC and Comparison Among Different Extraction Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 100-103. DOI： 10.13422/j.cnki.syfjx.2014220100.

摘要

目的: 建立HPLC同时测定长白山牛皮杜鹃中芦丁、槲皮素及山柰酚含量的方法

并比较不同提取方法对其含量的影响.方法: 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm

5 μm);乙腈-0.1%磷酸水溶液进行梯度洗脱;检测波长为360 nm

流速为0.8 mL ·min-1

柱温为30 ℃.结果: 芦丁、槲皮素、山柰酚的在一定范围内线性关系均良好;平均加样回收率分别为99.9%(RSD 1.2%)

99.2%(RSD 2.8%)

101.8%(RSD 2.3%).结论: 该方法简便、快速、准确可靠.3种不同提取方法中以超声提取法所得黄酮类含量均最高.可用于长白山牛皮杜鹃的质量控制.

Abstract

To establish an HPLC method for the simultaneous determination of rutin

quercetin and populnetin in Rhododendron chrysanthum from Changbai mountain and compare their contents by different extractive methods. The HPLC equipped with the Agilent Eclipse XDB-C18column (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile and 0.1% phosphoric acid with gradient elution. The detection wave length was set at 360 nm and the flow rate was 0.8 mL · min-1

and the temperature was set at 30 ℃. The linear ranges of rutin

quercetin and populnetin were good

the average recoveries were 99.9%(RSD 1.2%)

99.2%(RSD 2.8%)

101.8%(RSD 2.3%)

respectively. The method is simple

quick

accurate and repeatable for quantitative analysis. The supersonic extraction showed the highest content of flavonoids among the three extractive methods. The method can be used for quality control of R. chrysanthum from Changbai mountain.

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Related Institution

Department of Pharmacy,Leshan Vocational Technical College

Guangxi Traditional Chinese Medical University

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