WANG Yan, PEI Miao-rong. Neurotransmitter Influences of Component Comptibility Sini Tang to Hypothyroidism[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 139-141.
DOI:
WANG Yan, PEI Miao-rong. Neurotransmitter Influences of Component Comptibility Sini Tang to Hypothyroidism[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 139-141. DOI: 10.13422/j.cnki.syfjx.2014220139.
Neurotransmitter Influences of Component Comptibility Sini Tang to Hypothyroidism
目的: 研究组分配伍四逆汤对甲状腺功能减退症脑神经递质的影响.方法: 将96只大鼠随机分为空白组、模型组、优甲乐阳性对照组(0.01 g ·kg-1)
组分配伍四逆汤高、中、低剂量组(9.6
4.8
2.4 g ·kg-1).除空白组外
其他组连续灌胃丙基硫氧嘧啶(PTU)溶液0.01 g ·kg-1 15 d 造甲减模型
用组分配伍四逆汤高、中、低剂量和优甲乐对此动物模型进行治疗
连续给药1个月
治疗同时隔日给予1%PTU维持甲减状态
实验结束后
取脑组织
做10%脑匀浆
用酶联免疫吸附法测去甲肾上腺素(NA)
5-羟色胺(5-HT)和多巴胺(DA)浓度.结果: 模型组与空白组相比
其NA浓度明显降低(P<0.05)
5-HT浓度显著升高(P<0.01)
DA浓度明显降低(P<0.05).与模型组相比
四逆汤高、中剂量、阳性药组
NA浓度有显著升高(P<0.01);四逆汤低、中、高剂量组、阳性对照组
5-HT浓度明显降低(P<0.05
P<0.01);四逆汤高、中剂量、阳性药组
DA浓度显著升高(P<0.01).结论: 四逆汤对甲状腺功能减退症脑内神经递质有一定的调节作用.
Abstract
Objective:To study the brain neurotransmitter influences of component comptibility of Sini Tang(CCSNT) to hypothyroidism. Method: The hypothyroidism mode was made by 1% propylthiouracil (PTU) solution 15 days
treat the animal which has hypothyroidism with CCSNT of high
middle
low dose (9.6
4.8
2.4 g · kg-1
ig) and positive drugs Euthyrox 0.01 g · kg-1
ig
at the same time the mice should give the PTU(0.01 g · kg-1
ig);after the end of the experiment
take the brain and make 10% brain homogenates
use the enzyme-linked immunosorband assay (ELISA) kit to measure the norepinephrine(NA)
5-hydroxytryptaphane(5-HT)and dopamine(DA) concentration. Result: Compared with the blank group
in the model group the concentration of NA and DA are decreased(P<0.05)
concentrations of 5-HT is significantly increased (P<0.01). Compared with model group
the concentration of NA and DA of CCSNT high dose
middle dose and the Euthynx group
are significantly increased (P<0.05);the 5-HT level of all the treatment group are decreased(P<0.05). Conclusion: CCSNT has same effect of Sini Tang to neurotransmitters of hypothyroidism.
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