Objective:Artificial cultivation and anti-tumor effects of polysaccharides extracted from the fruit bodies of Lactarius volemus were studied. Method: The polysaccharides of L. volemus were extracted with boiling water. The proliferation inhibition of L. volemus polysaccharides on K562 cells was adopted by 4

5-dimethylthiazo(-2-yl) -2

5-diphenyl-tetrazolium bromide (MTT) method. The tumor apoptosis was investigated by scanning electron microscope(SEM) and agarose gel electrophoresis. Result: Artificial cultivation of L. volemus was achieved successfully. The growth of L. volemus mycelium was stimulated by carrots and pine roots

and the fruiting bodies were obtained in the third year. Polysaccharides of L. volemus had some anti-tumor activity which inhibition effect was strengthened significantly with increasing polysaccharides concentration. The maximum inhibition rate was reached at 88.2% with concentration of L. volemus polysaccharides at 100 mg · L-1 after 72 h. We found L. volemus polysaccharides promoted K562 tumor cells apoptosis that cell morphology contraction

significant reduction in microvilli by SEM. Meanwhile DNA ladder was appeared by agarose gel electrophoresis

which strength enhanced with increasing concentration of L. volemus polysaccharides. Conclusion: L. volemus may be a potential source for medicinal and edible mushrooms.