Objective:To determine the protective effect of total flavones from Clematis filamentosa (TFCF) on myocardial ischemia (MI). Method: Thirty-six rats were randomly divided into the sham operation group

model control group

compound danshen tablet group (250 mg · kg-1)

low-

moderate- and high-dose TFCF groups (8

16

32 mg · kg-1). MI was induced in rats by ligation of left anterior descending coronary artery except for rats in the sham operation group.Changes of segment ST of electrocardiogram

myocardial enzyme activity

and myocardial infarction area were observed. Thirty mice were randomly divided into the normal saline group

compound danshen tablet group (360 mg · kg-1)

low-

moderate- and high-dose TFCF groups (12

24

48 mg · kg-1). Hypoxia tolerance test was performed by placing the mice into the closed bottle and their survival time was measured. Result: Compared with the model control group

TFCF could reduce ST segment elevation at different time points (P<0.05)

decrease the serum levels of aspartate transaminase(AST)

lactate dehydrogenase(LDH)

and creatine kinase-MB(CK-MB) (P<0.05

P<0.01)

and reduce the rate of MI in acute myocardial ischemia rats (P<0.01). A certain dose-effect relationship was observed. TFCF also could remarkably prolong the mouse's survival time in hypoxia test (P<0.05). No significant difference was found between TFCF and compound danshen tablet. Conclusion: TFCF is proved to have an obvious protective effect on the experimental MI model.