Effects of Two Kinds of  Flavonoids on Osteoblast OPG/RANKL and Osteogenic Transcription Factor

LAN Bo; LIU Ting; XIE Yu-min; WANG Ai-min; LI Yong-jun

doi:10.13422/j.cnki.syfjx.2014220180

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Effects of Two Kinds of Flavonoids on Osteoblast OPG/RANKL and Osteogenic Transcription Factor

更新时间：2024-01-04

- Effects of Two Kinds of Flavonoids on Osteoblast OPG/RANKL and Osteogenic Transcription Factor
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 180-183(2014)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2014220180
  CLC： R285
- Published：2014
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LAN Bo, LIU Ting, XIE Yu-min, et al. Effects of Two Kinds of Flavonoids on Osteoblast OPG/RANKL and Osteogenic Transcription Factor[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 180-183.
DOI：

LAN Bo, LIU Ting, XIE Yu-min, et al. Effects of Two Kinds of Flavonoids on Osteoblast OPG/RANKL and Osteogenic Transcription Factor[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 180-183. DOI： 10.13422/j.cnki.syfjx.2014220180.

摘要

目的: 研究杜仲黄酮类化合物紫云英苷和黄芩素对成骨细胞特异性转录因子Osterix及破骨细胞抑制因子与核因子κB受体活化因子配体比值(OPG/RANKL)的影响.方法: 采用MC3T3-E1 Subclone 14成骨细胞

不同浓度紫云英苷和黄芩素和维生素D3组加入质量浓度为30 mg ·L-1作用细胞24 h后

用MTT法检测细胞增殖情况

成骨细胞接种于24孔板后

空白组加入培养基

给药组分别加入质量浓度为80 mg ·L-1(高浓度)

40 mg ·L-1(中浓度)

7.5 mg ·L-1(低浓度)的含紫云英苷或黄芩素

作用24 h后

ELISA法检测钙离子活性

蛋白免疫印迹法检测Osterix

OPG以及RANKL的蛋白表达水平.结果: 与空白组相比

紫云英苷和黄芩素可后明显促进MC3T3-E1 Subclone 14成骨细胞的增殖(P<0.05);明显上调OPG和Osterix的表达(P<0.05)

同时能降低RANKL的表达(P<0.05).结论: 杜仲黄酮类化合物紫云英苷和黄芩素能促进MC3T3-E1 Subclone 14成骨细胞增殖

并上调Osterix和OPG/RANKL.

Abstract

Objective:To investigate the effects of Eucommia ulmoides flavonoids astragalin and baicalein on the expression of osteoblast specific transcription factor Osterix and the ratio of osteoproteger and receptor activator of nuclear factor kappa B ligand (OPG/RANKL). Method: MC3T3-E1 Subclone 14 osteoblasts were cultured with different concentrations of astragalin

baicalein and vitamin D3 for 24 h. MTT detection method was used to detect cell proliferati. The expression of Ca2+ was detected by ELISA. The expressions of OPG

RANKL

OST were detected by Western blotting. Result: Compared with the control group

astragalin and baicalein can significantly promote the proliferation of MC3T3-E1 Subclone 14 (P<0.05)

increase the expression of OPG and Osterix obviously (P<0.05)

and reduce the expression of RANKL (P<0.05). Conclusion: It shows that astragalin and baicalein from E. ulmoides flavonoids can promote MC3T3 E1 Subclone 14 osteoblast proliferation

and raised Osterix and OPG/RANKL.

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