Objective: To investigate feasibility of concentration of Scutellariae Radix decoctions by integrated technology of ultrafiltration and reverse osmosis. Method: Scutellariae Radix decoctions was treated in turn by centrifuging

ultrafiltration

reverse osmosis.HPLC was adopted to compare main chemical composition of samples before and after treatment

mobile phase was methanol(A)-0.05% phosphoric acid(B) for gradient elution(0-35 min

40%A;35-75 min

60%A)

detection wavelength was set at 280 nm.In addition

NaOH solution

HCl solution

sodium dodecyl benzene sulfonate solution and pure water are respectively used to clean membrane fouling. Result: After concentrated 4 hours

concentration ratio of pretreated decoctions was 3.2 times and flux attenuation was 67.64%;concentration ratio of decoctions without pretreatment was 1.6 times and flux attenuation was 85.35%.Contents of baicalin before and after being concentrated were 1.03 g· L-1 and 8.87 g· L-1

respectively.Similarities of all test solutions were more than 0.99

it showed that samples before and after concentration had good similarity.After cleaned by different cleaning fluids

flux recovery rates of reverse osmosis were 87.73%

81.50%

67.65%

93.35%

so taking pure water to clean membrane fouling. Conclusion: Integrated technology of ultrafiltration and reverse osmosis has advantages of high retention rate of chemical ingredients and high concentration efficiency

which can be used to concentrate Scutellariae Radix decoctions.