Objective: To explore effects of processing and usage on dissolution of water

alcohol soluble total components

sinapine and fatty oil from drug pairs of Raphani Semen and Armeniacae Semen Amarum. Method: The content of sinapine was determined by HPLC

mobile phase was acetonitrile-0.1% phosphoric acid solution(15: 85) and detection wavelength was set at 326 nm;contents of total components in water

alcohol-soluble extract and fatty oil were determined by weight method.Composition and content of fatty acids in Armeniacae Semen Amarum was analyzed by GC-MS with DB-5 quartz capillary column(0.25 mm×30 m)

temperature of injection port and gasification room were 230℃ and 270℃

respectively;split ratio was 50: 1

programmed temperature(100-180℃

10℃ · min-1;180-200℃

4℃ · min-1;200-220℃

1℃ · min-1;220-270℃

5℃ · min-1)

MS conditions for EI ion source

70 eV and scanning range of m/z 30-500. Result: Total composition was easy to dissolving out by processing

dissolution of water

alcohol soluble ingredients and sinapine were 9.61%

141.03% and 20.00%

while increase were 0.36-1.85

38.41-62.06

1.40-2.60 times after being crushed

respectively;dissolution between single-decoction and mixed-decoction had no significant differences

dissolution of sinapine reduced 45.16%-68.75% after mixed-decoction. Conclusion: If seeds Chinese medicine not shattered

it do not suitable for extracting by alcohol or wine

crushing can significantly increase solubility of these measured components

mixed-decoction of Raphani Semen and Armeniacae Semen Amarum againsts dissolution of sinapine.Processing in accordance and reasonable application must be combined to ensure curative effect of traditional Chinese medicine.