Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of  by Response Surface Methodology

XIE Yu-feng; CHEN Ri-jia; YAN Chun-yan

doi:10.13422/j.cnki.syfjx.2014230032

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Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 23, Pages: 32-36(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014230032
  CLC： R284.1
- Published：2014
- 稿件说明：
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XIE Yu-feng, CHEN Ri-jia, YAN Chun-yan. Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 32-36.
DOI：

XIE Yu-feng, CHEN Ri-jia, YAN Chun-yan. Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 32-36. DOI： 10.13422/j.cnki.syfjx.2014230032.

摘要

目的: 优选番石榴叶中总三萜超声提取工艺. 方法: 以总三萜提取率为指标

在单因素试验基础上

通过响应面法考察乙醇体积分数、料液比、超声功率对总三萜提取率的影响.采用UV测定总三萜含量

检测波长540 nm. 结果: 最佳提取工艺条件为加60倍量70%乙醇于150 W

50℃ 超声提取2次

每次30 min.总三萜提取率7.93%

与理论值7.86%偏差较小. 结论: 通过响应面分析法优选番石榴叶总三萜提取工艺可行性较好

为该植物部位的开发与利用提供实验依据.

Abstract

Objective: To optimize extraction process of total triterpenoids from leaves of Psidium guajava. Method: Considering yield of total triterpenoids as index

based on single factor experiments

response surface methodology was adopted to optimize extraction process by taking ethanol concentration

liquid-solid ratio and ultrasonic power as factors.UV was employed to determine the content of total triterpenoids at 540 nm. Result: Optimum technological conditions were as follows:extracted twice with 60 times the amount of 70% ethanol at 150 W and 50℃ for 30 min of per time;yield of total triterpenoids was 7.93%

whose deviation was small by comparing with the theoretical value of 7.86%. Conclusion: Response surface analysis used in optimization of total triterpenoids is feasible

this study can provide experimental basis for development and utilization of this plant part.

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