Optimization of Enzymatic Extraction Technology of Cuscutae Semen

ZHOU Rong; WANG Luo-lin; XU Wen-jie; XIE Yuan; TU Yao-sheng; CHENG Xue-ren

doi:10.13422/j.cnki.syfjx.2014230033

您当前的位置：

首页 >

文章列表页 >

Optimization of Enzymatic Extraction Technology of Cuscutae Semen

更新时间：2024-01-04

- Optimization of Enzymatic Extraction Technology of Cuscutae Semen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 22, Pages: 33-36(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014230033
  CLC：
- Published：2014
- 稿件说明：
移动端阅览
ZHOU Rong, WANG Luo-lin, XU Wen-jie, et al. Optimization of Enzymatic Extraction Technology of Cuscutae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 33-36.
DOI：

ZHOU Rong, WANG Luo-lin, XU Wen-jie, et al. Optimization of Enzymatic Extraction Technology of Cuscutae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(22): 33-36. DOI： 10.13422/j.cnki.syfjx.2014230033.

摘要

目的: 优选菟丝子的酶法提取工艺

以提高菟丝子中金丝桃苷及总黄酮的提取率.方法: 以金丝桃苷提取量为指标

通过单因素试验考察酶种类、酶用量、酶解时间、酶解pH及温度对酶解效果的影响;以金丝桃苷与总黄酮提取量的综合评分为指标

采用正交试验考察酶用量、酶解pH及酶解温度对酶解工艺的影响.采用HPLC测定金丝桃苷含量

流动相乙腈-0.1%磷酸溶液(17:83)

检测波长360 nm.结果: 选择纤维素酶

最佳提取工艺为酶用量2.0%

酶解时间2.0 h

酶解pH 4.0

酶解温度45 ℃;金丝桃苷、总黄酮提取量分别为1.408

26.966 mg ·g-1.结论: 优选的酶法提取工艺稳定合理

适用于菟丝子的工业生产.

Abstract

Objective:To optimize enzymatic extraction process of Cuscutae Semen

in order to increase extracting rates of hyperin and total flavonoids. Method: With the content of hyperin as index

effects of enzyme species

enzyme dosage

enzymolysis time

pH and temperature on enzymatic extraction process were investigated by single factor test.With composite score of extracting amounts of hyperin and total flavonoids as index

orthogonal test was used to optimize enzymatic extraction process with enzyme dosage

enzymolysis pH and temperature as factors.HPLC was employed to determine the content of hyperin with mobile phase of acetonitrile-0.1% phosphoric acid solution(17: 83) and detection wavelength at 360 nm. Result: Cellulase was selected

its optimum extraction technology was as follows:enzyme dosage 2.0%

enzymolysis time of 2 hours

enzymatic hydrolysis of pH 4.0

enzymolysis temperature at 45 ℃;extracting amounts of hyperin and total flavonoids were 1.408

26.966 mg · g-1

respectively. Conclusion: This optimized extracting process is stable

reasonable and applicable to industrial production of Cuscutae Semen.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Feasibility Analysis of UPLC-assisted for Optimization of Elution Conditions on Macroporous Resin

Effect of Shenge Bushen Capsules and Its Polysaccharides and Flavonoids on Precocious Puberty in Young Mice

Mechanism of Cuscutae Semen to Control Premature Ovarian Failure Based on Lipid and Atherosclerosis Pathways

Effect of Cuscutae Semen on Learning and Memory and N6-methyladenosine Modification in Hippocampus of Offspring of Fear-damaged Pregnant Rats

Effect of Total Flavonoids in Flower of Paeonia suffruticosa on Uric Acid in Rats with Hyperuricemia

Related Author

LI Mu-chun

Alifu·

Abudu

HE Fei

Ayigl·

Taxi

SUN Hong

LEI Fan

Related Institution

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

Maternal and Child Health Hospital of Hubei Province

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰