XIONG Yan, ZHANG Jie, SHI Jun, et al. Effects of Different Processing Methods on Contents of Paeonol,Paeoniflorin, Total Flavonoids and Total Polysaccharides in Moutan Cortex[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 40-43.
DOI:
XIONG Yan, ZHANG Jie, SHI Jun, et al. Effects of Different Processing Methods on Contents of Paeonol,Paeoniflorin, Total Flavonoids and Total Polysaccharides in Moutan Cortex[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 40-43. DOI: 10.13422/j.cnki.syfjx.2014230040.
Effects of Different Processing Methods on Contents of Paeonol,Paeoniflorin, Total Flavonoids and Total Polysaccharides in Moutan Cortex
Objective: To discuss effects of different processing methods on contents of main components
in order to provide a reference for comprehensive evaluation of efficacy and quality of Moutan Cortex. Method: RP-HPLC was established for determining contents of paeonol and paeoniflorin
mobile phases were methanol-water(55: 45) and acetonitrile-0.1% phosphoric acid(14: 86)
detection wavelengths were 274 nm and 230 nm.Aluminum nitrate chromogenic method was adopted to determine the content of total flavonoids with detection wavelength at 508 nm;sulphuric acid-phenol colorimetric method was used to determine the content of total polysaccharides at 485 nm. Result: Contents comparison of paeonol
paeoniflorin
total flavonoids and total polysaccharides were in order of stir-frying products with wine>crude products>fried yellow products>fried coke products>carbonizing products by stir-frying
fried yellow products>crude products>stir-frying products with wine>fried coke products>carbonizing products by stir-frying
stir-frying products with wine>crude products>fried yellow products>fried coke products>carbonizing products by stir-frying
crude products>stir-frying products with wine>fried yellow products>fried coke products>carbonizing products by stir-frying
respectively. Conclusion: Determination of multi-index ingredients is benefit to monitor quality of processed products of Moutan Cortex and interpret its processing mechanism.