XIE Zhen, ZHANG Long, CHEN Yong, et al. Comparative on Characteristic Chromatogram of Different Processing Methods of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 56-59.
DOI:
XIE Zhen, ZHANG Long, CHEN Yong, et al. Comparative on Characteristic Chromatogram of Different Processing Methods of [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 56-59. DOI: 10.13422/j.cnki.syfjx.2014230056.
Comparative on Characteristic Chromatogram of Different Processing Methods of
Objective: The aim of this study was to establish a sensitive and specific HPLC method for quality control of Ilex hainanensis. Method: HPLC method was applied for quality assessment of I. hainanensis. HPLC analysis was performed on a phenomenon C18 column (4.6 mm×250 mm
5 μm).The mobile phase was consisted of acetonitrile (solvent B) and watercontaining 0.1% phosphoric acid (solvent A) at a constant flow rate of 1.0 mL· min-1.An increasing linear gradient of solvent B was used. The injection volume was 10 μL. The column temperature was set at 25℃. The chromatograms were monitored at 330 nm. Result: In I. hainanensis obtained from different processing methods
there were 9 characteristic peaks identified. It was established from its prepared samples of I. hainanensis. Conclusion: The characteristic chromatogram of I. hainanensis with high specificity can be used to control its quality as well as the uniformity of processing products.
Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.
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