HPLC Fingerprints of  in Guangxi

HUANG Hong-hong; ZHEN Han-shen; LIU Xian-fu

doi:10.13422/j.cnki.syfjx.2014230060

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HPLC Fingerprints of in Guangxi

更新时间：2024-01-04

- HPLC Fingerprints of in Guangxi
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 23, Pages: 60-62(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014230060
  CLC： R284.1
- Published：2014
- 稿件说明：
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HUANG Hong-hong, ZHEN Han-shen, LIU Xian-fu. HPLC Fingerprints of in Guangxi[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 60-62.
DOI：

HUANG Hong-hong, ZHEN Han-shen, LIU Xian-fu. HPLC Fingerprints of in Guangxi[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 60-62. DOI： 10.13422/j.cnki.syfjx.2014230060.

摘要

目的: 采用HPLC研究广西不同产地毛果算盘子的指纹图谱. 方法: 采用Hypersil C18柱(4.6 mm×250 mm

5 μm)

流动相甲醇(A)-0.2%磷酸(B)梯度洗脱(0~7 min

10%A;7~15 min

10%~22%A;15~20 min

22%~30%A;20~30 min

30%~40%A;30~45 min

40%A)

检测波长270 nm

柱温20℃

流速1.0 mL·min-1

分析时间45 min.分析了10批毛果算盘子药材的HPLC指纹图谱.应用中药色谱指纹图谱相似度评价系统(2004A版)计算相似度. 结果: 建立了广西产毛果算盘子的指纹图谱

且相似度较好

根据相似度分析确定了9个构成毛果算盘子药材指纹图谱的特征峰. 结论: 该方法简单、稳定、重复性好

可用于毛果算盘子药材的质量控制.

Abstract

Objective: The study aimed to establish the chromatographic fingerprint of Glochidion eriocarpum by HPLC. Method: The fingerprint was investigated using Hypersil C18 (4.6 mm×250 mm

5μm) column. The mobile phase was consisted of methanol (A) and 0.2% phosphoric acid (B)

in gradient elution for 0-7 min

10%A;7-15 min

10%-22%A;15-20 min

22%-30%A;20-30 min

30%-40%A;30-45 min

40%A. The detection wavelength was set at 270 nm

and column temperature was set at 30℃

The flow rate was 1 mL· min-1. The fingerprints of 10 batches of G. eriocarpum were analyzed in 45 min. The similarity was analyzed by similarity evaluation system for chromatographic fingerprint of TCM (version 2004 A). Result: The HPLC fingerprint of Guangxi G. eriocarpum was obtained with good similarity. Conclusion: The method was simple

reproducible and can be used in quality control for G. eriocarpum.

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