ZHU Xiao-ning, WANG Jing, MI Shao-ping, et al. Effects of Qutan Huoxue Decocotion on Expression of Aquaporin 9 in Nonalcoholic Fatty Liver Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 160-164.
DOI:
ZHU Xiao-ning, WANG Jing, MI Shao-ping, et al. Effects of Qutan Huoxue Decocotion on Expression of Aquaporin 9 in Nonalcoholic Fatty Liver Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 160-164. DOI: 10.13422/j.cnki.syfjx.2014230160.
Effects of Qutan Huoxue Decocotion on Expression of Aquaporin 9 in Nonalcoholic Fatty Liver Rats
Objective: To observe the effect of Qutan Huoxue decocotion (QTHXD) on the expression of aquaporin 9 (AQP9) in the nonalcoholic fatty liver rats
and discuss the mechanism. Method: Sixty SD rats were randomly divided into 5 groups
12 each group:the blank control group
model control group
QTHXD low
medium and high dose groups(6
12
16 g· kg-1)
all rats except balnk congtrol group were fed high fat diet for 10 weeks to establish nonalcoholic fatty liver disease(NAFLD) model. After modelingg
the corresponding drugs were ig giventwice a day
for 2 weeks.HE
ELISA and RT-PCR were used to observe the liver pathology changes
total cholesterol(TC)
triglycerides(TG) and the AQP9 mRNA expression in liver cells. Result: ① AQP9 expression in liver cells of NAFLD model group compared with blank control group was raised.
but decreased in three QTHXD dose 12 g· kg-1group was lower than the other two groups.②TC and TG in three QTHXD dose groups deacreased
16 g· kg-1group was lower than the other two groups.③Hepatic steatosis in the QTHXD different dose groups was reduced compared with NAFLD model group
and hepatic steatosis in 16 g· kg-1 group reduced more markedly than the other two groups. Conclusion: QTHXD could regulate AQP9 expression of liver cells in NAFLD model rats
reduce TC and TG and improve the degree of hepatic steatosis.
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