LI Yu-feng, ZHANG Bi-yan, LAI Yun, et al. Effects of Banxia Xiexin Tang on Intestinal Immune Function in Fluorouracil-induced Diarrhea Mice[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 180-184.
DOI:
LI Yu-feng, ZHANG Bi-yan, LAI Yun, et al. Effects of Banxia Xiexin Tang on Intestinal Immune Function in Fluorouracil-induced Diarrhea Mice[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 180-184. DOI: 10.13422/j.cnki.syfjx.2014230180.
Effects of Banxia Xiexin Tang on Intestinal Immune Function in Fluorouracil-induced Diarrhea Mice
Objective: To study the effect of Banxia Xiexin Tang on intestinal immune function in fluorouracil-induced diarrhea mice
and to reveal the therapeutic mechanism on chemotherapy-induced diarrhea. Method: The fluorouracil(40 mg· kg-1) was intraperitoneal injected for 6 days to establish the diarrhea mice model. Sixty ICR mice were randomized into 6 groups:normal group
model group
montmorillonite powder group(1.17 g·kg-1)
Banxia Xiexin Tang(2.5
1.25
0.625 g· kg-1) group
administrated by oral gavage for 2 day before modeling
the intervention period lasted 11 days. Then we observed the changes of daily activity status
food intake
body weight
diarrhea
measured the quality of the thymus and spleen
observed the pathological changes of ileal tissue
measured the expression of intestinal immunoglobulin A (IgA)
vasoactive intestinal peptide (VIP)
interleukin-15(IL-15)by ELISA method. Result: Compared to normal group
the diarrhea of model group mice was aggravated and the weight
thymus and spleen index were decreased
the levels of IgA and VIP were decreased
whereas the IL-15 was increased.Compared to model group
Banxia Xiexin Tang could significantly inhibit body weight loss
diarrhea and spleen
thymus reduction induced by fluorouracil in mice. Banxia Xiexin Tang could increase the levels of IgA and VIP and decrease IL-15 expression (P<0.05
P<0.01). Conclusion: Banxia Xiexin Tang could prevent the fluorouracil induced diarrhea by up-regulating the expression of IgA
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Related Author
LI Jing-tao
SHI Jie
YAN Shu-guang
HUI Yi
ZHANG Xin-yue
ZHENG Xu-rui
Yue-fang XU
Zhao-zhao HUA
Related Institution
College of Basic Medicine,Shaaxi University of Chinese Medicine
Affiliated Hospital,Shaaxi University of Chinese Medicine
The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(TCM)
The Second Affiliated Hospital of Guizhou University of TCM