Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue

LIU Bin; WANG Bing-sheng; LIU Xiu-fang; ZHANG Hai

doi:10.13422/j.cnki.syfjx.2014230185

您当前的位置：

首页 >

文章列表页 >

Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue

更新时间：2024-01-04

- Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 23, Pages: 185-189(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014230185
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
LIU Bin, WANG Bing-sheng, LIU Xiu-fang, et al. Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 185-189.
DOI：

LIU Bin, WANG Bing-sheng, LIU Xiu-fang, et al. Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 185-189. DOI： 10.13422/j.cnki.syfjx.2014230185.

摘要

目的: 检测益气活血方干预前后大鼠放射性肺损伤组织基质金属蛋白酶2(matrix metalloproteinase-2

MMP2)和基质金属蛋白酶抑制剂2(tissue inhibitor of matrix metalloproteinase

TIMP2)表达的影响

了解该方剂的作用机制. 方法: 健康成年雌性SD大鼠120只

随机分为模型组30只、照射加益气活血方高剂量组30只、照射加益气活血方低剂量组30只、空白组30只.益气活血方高、低剂量组大鼠受照当天开始

用药量20

10 g·kg-1

每次ig益气活血方剂5 mL·kg-1

2次/d;模型组、空白组每次ig蒸馏水5 mL·kg-1

2次/d.4组大鼠分别于照射后第4

20周末随机抽取6只

处死并取其右肺组织保存.分别用反转录PCR技术(RT-PCR)

蛋白质印迹法(Western-blotting)检测MMP-2／TIMP-2 mRNA及蛋白表达的动态变化. 结果: 模型组MMP-2 mRNA及蛋白表达于第12周达高峰

而TIMP-2 mRNA及蛋白表达在照射后第4周至20周期间出现逐渐升高的趋势

各时间点模型组与空白组相比均具有明显差异(P<0.01).益气活血方高剂量组与模型组相比

各mRNA及蛋白表达趋势与模型组相似

MMP-2 mRNA及蛋白表达在12周前低于模型组

差异有统计学意义(P<0.05

P<0.01).益气活血方低剂量组各项指标均有变化

趋势与模型组类似

差异没有统计学意义. 结论: 益气活血方高剂量在放射性肺损伤的各个时间段

从转录及翻译层面对MMP-2/TIMP-2的表达进行调整

使MMPs/TIMPs趋于平衡

从而减轻放射性肺损伤

这可能是该药防治放射性肺损伤的分子生物学机制.

Abstract

Objective: To study the effect of the Yiqi Huoxue recipe on expression of the matrix metalloproteinase-2(MMP-2)/tissue inhibitor of matrix metalloproteinase(TIMP-2) in radiation-induced lung injury model rats

in order to know the mechanism of this recipe. Method: One hundred and twenty female rats were divided into four groups at random:namely simple exposure group(model group

n=30)

radiational Yiqi Huoxue recipe high-dose group and the radiational Yiqi Huoxue recipe low-dose group(n=30)

and blank control group(control group

n=30). Six rats were selected randomly from the four groups each time at the 4th

6th

8 th

12th

26th weekend after first radiation

those rats were executed immediately after intraperitoneal anesthesia

took the right lung tissue of them and saved in right way. Reverse transcription PCR(RT- PCR) was used to detect the dynamic change of mRNA expression of MMP-2

TIMP-2 in each time point.Western-blotting was used to detect the dynamic change of protein expression of MMP-2

TIMP-2 in each time point. Result: The expression of MMP-2 mRNA and protein appeared a first increased and then decline trend from 4thweekend to 20th weekend after irradiation

and the peak was in 12th.The expression of TIMP-2 mRNA and protein appeared a rising tend from 4thweekend to 20th weekend after irradiation. The mRNA and protein expression trend is similar to Yiqi Huoxue recipe high-dose group and model group

but they have different change in different time point. In Yiqi Huoxue recipe low-dose group the indicators changed with no statistical significance. Conclusion: High-dose Yiqi Huoxue recipe can adjust the expression of MMP-2/TIMP-2 from the transcription and translation level in each period of radioactive lung injury

making so as to reduce the radioactive lung injury. This may be the molecular biology mechanism of the drug in prevention and control of radioactive lung injury.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Yiqi Huoxue Recipe on expression of ET-1/γ-IFN in Rats with Radiation-induced Lung Injury

Regulatory Mechanism of Radiation Therapy for Lung Cancer with Traditional Chinese Medicine： A Review

Role of Cytokines and Related Signaling Pathways in Intervention of Chinese Medicine in Radiation-induced Lung Injury： A Review

Effect of Yiqi Huoxue Recipe on Oxidative Stress Response in Rats with Cerebral Ischemia Injury Based on Nrf2/HO-1 Signaling Pathway

Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome

Related Author

LIU Xin

LI Feng-yu

LU Tingting

WANG Yu

ZHANG Lu

TANG Leilei

YU Dongdong

JIANG Wenjing

Related Institution

Institute of Traditional Chinese Medicine Respiratory Disease Prevention and Treatment， Anhui Academy of Chinese Medicine

Key Laboratory of Xin'an Medicine， Ministry of Education， Anhui University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

School of Integrated Chinese and Western Medicine，Anhui University of Chinese Medicine

School of Chinese Medicine， School of Integrated Chinese and Western Medicine， Nanjing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰