Stability of Preparation Process of Total Flavonoids and Total Saponins from Ziziphi Spinosae Semen

ZHAO Qi-duo; SHU Le-xin; CAI Guang-zhi

doi:10.13422/j.cnki.syfjx.2014240027

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Stability of Preparation Process of Total Flavonoids and Total Saponins from Ziziphi Spinosae Semen

更新时间：2024-01-04

- Stability of Preparation Process of Total Flavonoids and Total Saponins from Ziziphi Spinosae Semen
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 27-29(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240027
  CLC： R284.1
- Published：2014
- 稿件说明：
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ZHAO Qi-duo, SHU Le-xin, CAI Guang-zhi. Stability of Preparation Process of Total Flavonoids and Total Saponins from Ziziphi Spinosae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 27-29.
DOI：

ZHAO Qi-duo, SHU Le-xin, CAI Guang-zhi. Stability of Preparation Process of Total Flavonoids and Total Saponins from Ziziphi Spinosae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 27-29. DOI： 10.13422/j.cnki.syfjx.2014240027.

摘要

目的: 考察酸枣仁总黄酮和总皂苷部位制备工艺的稳定性

为后续药效学评价的样品制备提供保证. 方法: 采用相同工艺条件制备10批酸枣仁总黄酮和总皂苷部位样品.选择斯皮诺素、白桦酯酸及酸枣仁皂苷A

B为指标成分

利用 ELSD/HPLC分析两部位样品的特征图谱

流动相乙腈(A)-水(B)梯度洗脱(0~10 min

12%~18%A;11~20 min

19%~23%A; 21~30 min

23%~34%A;31~40 min

35%~45%A;41~50 min

46%~100%A;51~60 min

100%~11%A;61~70 min

12%A)

漂移管温度40 ℃. 结果: 总黄酮部分的化学成分分布主要集中于前25 min

总皂苷部分化学成分集中在25 min后

总黄酮和总皂苷纯度均>50%

54.46 min处色谱峰确定为白桦酯酸. 结论: 制备酸枣仁总黄酮和总皂苷部位的工艺稳定性良好

可作为药效学试验的样品.

Abstract

Objective: Stability of preparation process of total flavonoids and total saponins from Ziziphi Spinosae Semen was investigated

in order to provide a guarantee for sample preparation of follow-pharmacodynamic evaluation. Method: Ten batches of total flavonoids and total saponins from Ziziphi Spinosae Semen were prepared with the same preparation process.Spinosin

betulinic acid

jujuboside A and jujuboside B were taken as index components

ELSD/HPLC was employed to analyze feature profiles of these two parts samples with mobile phase of acetonitrile(A)-water(B) for gradient elution(0-10 min

12%-18%A;11-20 min

19%-23%A;21-30 min

23%-34%A;31-40 min

35%-45%A;41-50 min

46%-100%A;51-60 min

100%-11%A;61-70 min

12%A) and drift tube temperature of 40 ℃. Result: Chemical compositions distribution of total flavonoids part focused on the fomer 25 min

but that of total saponins concentrated in after 25 min

purities of total flavonoids and total saponins were more than 50%

peak at 54.46 min was identified as betulinic acid. Conclusion: This preparation technology of total flavonoids and total saponins from Ziziphi Spinosae Semen is stable

which can be used as samples of pharmacodynamic experiment.

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