Optimization of Enrichment Process for Total Lignans from Arctii Fructus Residue After Subcritical Extraction

LU Ying-cai; GAO Ying; XIONG Shu-ping; LI Wei-min

doi:10.13422/j.cnki.syfjx.2014240030

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Optimization of Enrichment Process for Total Lignans from Arctii Fructus Residue After Subcritical Extraction

更新时间：2024-01-04

- Optimization of Enrichment Process for Total Lignans from Arctii Fructus Residue After Subcritical Extraction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 30-34(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240030
  CLC： R284.1
- Published：2014
- 稿件说明：
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LU Ying-cai, GAO Ying, XIONG Shu-ping, et al. Optimization of Enrichment Process for Total Lignans from Arctii Fructus Residue After Subcritical Extraction[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 30-34.
DOI：

LU Ying-cai, GAO Ying, XIONG Shu-ping, et al. Optimization of Enrichment Process for Total Lignans from Arctii Fructus Residue After Subcritical Extraction[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 30-34. DOI： 10.13422/j.cnki.syfjx.2014240030.

摘要

目的: 优选经亚临界萃取后牛蒡子药渣中总木脂素的富集工艺

为该药材资料的开发与利用提供参考. 方法: 以牛蒡子油得率、药渣中总木脂素含量为指标

通过单因素试验考察牛蒡子亚临界萃取溶媒.采用醇提水沉法富集牛蒡子药渣中木脂素类成分

通过单因素试验优选工艺条件.采用HPLC测定牛蒡子苷和牛蒡子苷元的含量

流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~5 min

30%~35%A;5~13 min

35%~45%A;13~15 min

45%~48%A;15~20 min

48%~53%A;20~29 min

53%~80%A;29~40 min

80%~100%A)

检测波长280 nm. 结果: 选择丁烷为萃取溶媒脱脂.最佳富集工艺为加8倍量70%乙醇回流提取3次

每次1 h

滤液减压回收至0.5 g·mL-1

按药渣量加12倍量水沉淀

煮沸回流60 min;总木脂素纯度>50%

转移率>80%. 结论: 醇提水沉法适用于牛蒡子中总木脂素部位的富集纯化

优选的工艺稳定可行.

Abstract

Objective: To optimize enrichment technology of total lignans from Arctii Fructus residue after subcritical extraction. Method: Taking yield of Arctii Fructus oil and the content of total lignans in residue as indexes

subcritical extraction solvent of Arctii Fructus was investigated by single factor test.Total lignans in residue was enriched by alcohol extraction and water precipitation method

process parameters was optimized by single factor tests.HPLC was employed to determine contents of arctiin and arctigenin with mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-5 min

30%-35%A;5-13 min

35%-45%A;13-15 min

45%-48%A;15-20 min

48%-53%A;20-29 min

53%-80%A;29-40 min

80%-100%A) and detection wavelength at 280 nm. Result: Butane was selected as extraction solvent for degreasing.Optimum enrichment process was as follows:reflux extracted thrice with 8 times the amount of 70% ethanol for 1 h each time

filtrate pressure recoveried to 0.5 g·mL-1

precipitation with 12 time the amount of water according to quantity of dregs

reflux boiling time of 60 minutes;purity of total lignans was more than 50%

transfer rate reached more than 80%. Conclusion: Alcohol extraction and water precipitation method is suitable for enrichment and purification of total lignans in Arctii Fructus

this optimized process is stable and feasible.

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