Objective: The aim of this study was to develop an HPLC method for the simultaneous determination of contents of scutellarin

protopine

apigenin and tetrahydropalmatine in Dengyan Granules. Method: The analysis was performed on a Zorbax Extend-C18 column (4.6 mm×250 mm

5 μm) in gradient elution (0-25 min

15%A;25-70 min

15%-65%A;70-75 min

65%-25%A) by using the mobile phase of acetonitrile(A)-0.3% ammonium acetate solution(B).The flow rate was 0.8 mL·min-1 and the column temperature was maintained at 30 ℃

the detection wavelengths were set at 280 nm (for scutellarin

protopine and tetrahydropalmatine)

337 nm (for apigenin)

respectively. Result: The calibration curves were linear within the range of 0.90-8.99 μg for scutellarin

0.73-7.35 μg for protopine

0.05-0.54 μg for apigenin and 0.14-1.37 μg for tetrahydropalmatine

respectively. The recoveries of scutellarin

protopine

apigenin and tetrahydropalmatine were 99.48% (RSD 0.8%)

98.17% (RSD 1.2%)

97.41% (RSD 1.2%) and 98.33% (RSD 0.5%)

respectively. Conclusion: The method can be used for simultaneous determination of contents of scutellarin

protopine

apigenin and tetrahydropalmatine in Dengyan Granules.