Determination of Multiple Index Components in Yinqiao Powder by UPLC

LIU Li-hui; XU Jian; ZHANG Yong-ping

doi:10.13422/j.cnki.syfjx.2014240067

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Determination of Multiple Index Components in Yinqiao Powder by UPLC

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- Determination of Multiple Index Components in Yinqiao Powder by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 67-70(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240067
  CLC： O657.72;R286
- Published：2014
- 稿件说明：
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LIU Li-hui, XU Jian, ZHANG Yong-ping. Determination of Multiple Index Components in Yinqiao Powder by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 67-70.
DOI：

LIU Li-hui, XU Jian, ZHANG Yong-ping. Determination of Multiple Index Components in Yinqiao Powder by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 67-70. DOI： 10.13422/j.cnki.syfjx.2014240067.

摘要

目的: 建立UPLC同时测定银翘散中绿原酸、甘草苷、橙皮苷、牛蒡子苷、连翘苷含量的方法. 方法: 采用UPLC系统

ACQUITY UPLC BEH Shield RP18色谱柱(2.1 mm×50 mm

1.7 μm)

甲醇-0.15%甲酸溶液梯度洗脱

流速0.25 mL·min-1

检测波长279 nm

柱温40 ℃

同时测量5种活性成分的含量. 结果: 绿原酸、连翘苷、牛蒡子苷、橙皮苷、甘草苷分别在0.018 76~0.043 77

0.002 37~0.011 85

0.011 21~0.056 06

0.001 34~0.006 71

0.001 71~0.012 38 μg线性关系良好(r≥0.999 1)

平均回收率分别为97.12%(RSD 0.9%)

101.40%(RSD 2.1%)

100.61%(RSD 1.6%)

100.79%(RSD 0.7%)

101.04% (RSD 1.7%). 结论: 该方法简便、快速、专属性强

可用于银翘散的质量控制.

Abstract

Objective: The aim of this study was to establish a method for the content determination of chlorogenic acid

liquiritin

hesperidin

arctiin and phillyrin in Yinqiao Powder. Method: UPLC system was performed on ACQUITY UPLC BEH Shield RP18 column(2.1 mm×50 mm

1.7 μm)at 40 ℃. The mobile phase was consisted of methanol-0.15% formic acid solution in gradient elution mode. The flow rate was set at 0.25 mL·min-1 and the detection wavelength was set at 279 nm. Result: Chlorogenic acid

phillyrin

arctiin

hesperidin and liquiritin had good linear relationship in the ranges of 0.018 76-0.043 77

0.002 370-0.011 85

0.011 21-0.056 06

0.001 34-0.006 71 and 0.001 71-0.012 38 μg

respectively. The corre-lation coefficients were all more than 0.999 1.The average recoveries were 97.12%(RSD 0.9%)

101.40%(RSD 2.1%)

100.61%(RSD 1.6%)

100.79%(RSD 0.7%)and 101.04%(RSD 1.7%)

respectively. Conclusion: The method is simple

rapid

specific

and can be used for quality control of Yinqiao powder.

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Related Institution

Shandong University of Traditional Chinese Medicine

Chongqing Three Gorges Medical College

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The People's Hospital Affiliated to Chongqing Three Gorges Medical College

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