Simultaneous Determination of Five Compounds in Xiongju Shangqing Tablets by HPLC

HAO Cheng-yi; GUO Shu-ying; FENG Bo

doi:10.13422/j.cnki.syfjx.2014240074

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Simultaneous Determination of Five Compounds in Xiongju Shangqing Tablets by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Five Compounds in Xiongju Shangqing Tablets by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 74-77(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240074
  CLC： R284.1
- Published：2014
- 稿件说明：
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HAO Cheng-yi, GUO Shu-ying, FENG Bo. Simultaneous Determination of Five Compounds in Xiongju Shangqing Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 74-77.
DOI：

HAO Cheng-yi, GUO Shu-ying, FENG Bo. Simultaneous Determination of Five Compounds in Xiongju Shangqing Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 74-77. DOI： 10.13422/j.cnki.syfjx.2014240074.

摘要

目的: 建立同时测定芎菊上清片中5种成分(绿原酸、盐酸小檗碱、黄芩苷、齐墩果酸、熊果酸)含量的方法. 方法: 采用高效液相色谱法

DIKMA Platisil ODS色谱柱(4.6 mm×250 mm

5 μm)

柱温30 ℃

流动相甲醇-0.1%磷酸水溶液梯度洗脱

检测波长分别为327 nm(绿原酸、盐酸小檗碱、黄芩苷)

210 nm(齐墩果酸、熊果酸)

流速1 mL·min-1. 结果: 芎菊上清片中的绿原酸、盐酸小檗碱、黄芩苷、齐墩果酸、熊果酸5种成分的线性范围分别为0.03~0.6

0.011 4~0.228

0.025~0.5

0.1~2

0.03~0.6 μg

平均加样回收率分别为99.57%(RSD 1.3%)

99.19%(RSD 0.8%)

99.14%(RSD 0.6%)

99.38%(RSD 1.2%)

98.92%(RSD 0.7%). 结论: 该法检测简便

稳定

可靠

是芎菊上清片质量控制的一个快捷有效的方法.

Abstract

Objective: To establish a method for simultaneous determination of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid in Xiongju Shangqing tablets. Method: The separation was performed on a DIKMA Platisil ODS column (4.6 mm×250 mm

5 μm) with gradient elution using methanol and 0.1% H3PO4 aqueous solution as mobile phase. The flow rate was 1 mL·min-1 and the detection wavelengths were 327 nm for chlorogenic acid

berberine and baicalin; 210 nm for oleanolic acid and ursolic acid

respectively. Result: Linear ranges of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid were 0.03-0.6

0.011 4-0.228

0.025-0.5

0.1-2

0.03-0.6 μg

respectively. Their average recoveries were 99.57%(RSD 1.3%)

99.19%(RSD 0.8%)

99.14%(RSD 0.6%)

99.38%(RSD 1.2%)

98.92%(RSD 0.7%)

respectively. Conclusion: The established HPLC method can be used to determine the contents of chlorogenic acid

berberine

baicalin

oleanolic acid and ursolic acid in Xiongju Shangqing tablets. The method can also be used as the standard for the quality control of Xiongju Shangqing tablets.

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