Objective: To study effect of Mieyou decoction on the expression of nuclear factor-κB(NF-κB) p65 protein and IL-8 in Helicobacter pylori(HP) related gastritis of spleen stomach damp heat syndrome mouse model. Method: Sixty BALB/c mice

half male and half female

were randomly divided into 6 groups:control group

model group

Mieyou decoction low

medium

high dose groups(6.2

12.4

24.8 g·kg-1)

gastric triad group(0.28 g·kg-1). The model was established by high fat feeding and heat box in eleventh days

on sixteenth days with HP was inoculated

and serum HP antibody was detected using colloidal gold method

on thirty-sixth days corresponding drugs were given for 14 days

the other groups received normal feeding. All the mice were sacrificed on fifty-third days. The general condition of mice was observed

and the food and water intake

body weight

body temperature changes were obsedved. HE staining was used to observe the gastric mucosa and integral opticallens

the expression of NF-κB p65 protein was assayed by immunohistochmistry serum IL-8 was determined by ELISA. Result: Compared with control group

the model group increased antral gastric mucosa of acute and chronic inflammation score and expression of NF-κB p65 protein and IL-8 (P<0.05);compared with the model group

the Mieyou decoction middle and high dose group decreased gastric triad antral gastric mucosa of acute and chronic inflammation score and expression of NF-κB p65 protein and IL-8(P<0.05). Conclusion: Mieyou decoction may exert its anti-inflammatory effects through inhibition of NF-κB p65 protein expression and IL-8.