Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD

SHANG Li-zhi; XIE Wen-ying; ZHANG Liang-zhi; PAN Xiao-li; HU Wen-hao; LIU Tan

doi:10.13422/j.cnki.syfjx.2014240168

您当前的位置：

首页 >

文章列表页 >

Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD

更新时间：2024-01-04

- Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 168-171(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240168
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
SHANG Li-zhi, XIE Wen-ying, ZHANG Liang-zhi, et al. Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 168-171.
DOI：

SHANG Li-zhi, XIE Wen-ying, ZHANG Liang-zhi, et al. Effect of Ailuo Kechuanning on Inflammatory Cytokines and Oxidative Stress of Lung Homogenates in Rats with COPD[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 168-171. DOI： 10.13422/j.cnki.syfjx.2014240168.

摘要

目的: 探讨爱罗咳喘宁方对慢性阻塞性肺疾病(COPD)肺部炎症及氧化损伤作用及机制. 方法: 采用脂多糖(LPS)加烟雾诱导 COPD大鼠模型

大鼠随机分为正常组、模型组、爱罗咳喘宁低、中、高剂量组.正常组、模型组灌胃生理盐水(15.52 mL·kg-1·d-1)

爱罗咳喘宁低、中、高剂量组分别灌胃(7.75

15.52

31.04) g·kg-1·d-1

连续14 d.用比色法、酶联免疫法(ELISA)分别测定大鼠肺匀浆中磷酸化p38MAPK(mitogen activatein kinase

p-p38MAPK)、髓过氧化物酶(MPO)活性

白介素-17(IL-17)和C反应蛋白(CRP)含量;并测定肺匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量. 结果: 与正常组比较

模型组p-p38MAPK和MPO活性、IL-17和CRP含量均显著升高(均P<0.01);SOD和GSH-Px活性均显著降低、MDA含量显著升高(均P<0.01);与模型组相比

爱罗咳喘宁中剂量组p-p38MAPK(P<0.05)、MPO(P<0.01)活性与IL-17

CRP(均P<0.01)含量均显著降低

SOD和GSH-Px活性均显著升高、MDA含量显著降低(均P<0.01). 结论: 爱罗咳喘宁对COPD有抗炎作用

其机制可能与抑制p-p38MAPK

MPO活性

减少IL-17的释放有关;爱罗咳喘宁对COPD有抗抗氧化损伤作用

其机制通过提高SOD和GSH-Px活性

减少MDA生成

保护肺组织的结构与功能.

Abstract

Objective: To study the effect of Ailuo Kechuanning on mitogen activatein kinase(p38MAPK)

myeloperoxidase(MPO)

interleukin-17(IL-17)

C-reactive protein(CRP)

and oxidative stress in rats with chronic obstructive pulmonary disease (COPD). Method: Lipopolysaccharide(LPS) smoke wasused to COPD rat model

the experimental animals were randomly divided into normal group

model group

Ailuo Kechuanning groups(7.75

15.52

31.04 g·kg-1·d-1)

The activity of p-p38MAPK and MPO was detected by colorimetric method

enzyme-linked immunosorbent assay was used to determine the change of IL-17 and C-reactive protein(CRP) levels.the concentration of MDA

the activity of SOD and glutethione peroxiadase(GSH-Px) were measured in lung homogenates of all the groups. Result: The activity of p-p38MAPK and MPO

the levels of IL-17 and CRP were higher significantly

the activity of SOD and GSH-Px were decreased

the level of MDA was higher significantly in lung homogenates of the model group. Compared with the normal control group. While the activity of p-p38MAPK and MPO

and the levels of IL-17 and CRP was decreased significantly.the activity of SOD and GSH-Px were higher significantly

and the level of MDA was decreased significantly in lung homogenates of the rats treated with Ailuo Kechuanning(15.52 g·kg-1·d-1)compared with the model group. Conclusion: Ailuo Kechuanning may improve the pulmonary function of COPD. The effects of Ailuo Kechuanning for treatment of COPD may inhibite the activity of p-p38MAPK and MPO

and decrease the levels of IL-17

CRP and MDA

lead to the anti-inflammatory and against oxidative damage.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Modified Erchentang on β₂AR/β-arrestin2 Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease

Connotation of Traditional Chinese Medicine's Protection of Pulmonary Microvascular Endothelial Cells in Prevention and Treatment of Chronic Obstructive Pulmonary Disease Based on "Lung Collateral Theory"

Traditional Chinese Medicine Intervention of Signaling Pathways in Chronic Obstructive Pulmonary Disease： A Review

Mechanism of Modified Erchentang on COPD Inflammation Based on TNF-α/TNFR1/RIPKs Pathway

Effect of Xuanfei Zhisou Prescription on IL-17 Signaling Pathway in COPD Model Rats

Related Author

Wen-ying XIE

Jun-yue WANG

Yong-sheng BAO

Li-zhi SHANG

Ke WU

Liang LI

Xiao-yan WANG

Xiao-hui CHEN

Related Institution

Henan University of Traditional Chinese Medicine

Key Laboratory of State Administration of Traditional Chinese Medicine （Cardio-Cerebral Vessel Collateral Disease）

State Key Laboratory for Innovation and Transformation of Luobing Theory

Graduate School， Hebei Medical University

First Affiliated Hospital， Heilongjiang University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰