Effect of Shugan Hewei Decoction on Expression of CGRP, of Medulla Oblongata,Spinal Cord and Gastric Mucosa in Depression Model Rats

LIU Song-lin; YUE Ying-ying; XING Ying; LI Ming-zhu; LI Jia-geng; FAN Xun

doi:10.13422/j.cnki.syfjx.2014240172

您当前的位置：

首页 >

文章列表页 >

Effect of Shugan Hewei Decoction on Expression of CGRP, of Medulla Oblongata,Spinal Cord and Gastric Mucosa in Depression Model Rats

更新时间：2024-01-04

- Effect of Shugan Hewei Decoction on Expression of CGRP, of Medulla Oblongata,Spinal Cord and Gastric Mucosa in Depression Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 24, Pages: 172-175(2014)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2014240172
  CLC： R285.5
- Published：2014
- 稿件说明：
移动端阅览
LIU Song-lin, YUE Ying-ying, XING Ying, et al. Effect of Shugan Hewei Decoction on Expression of CGRP, of Medulla Oblongata,Spinal Cord and Gastric Mucosa in Depression Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 172-175.
DOI：

LIU Song-lin, YUE Ying-ying, XING Ying, et al. Effect of Shugan Hewei Decoction on Expression of CGRP, of Medulla Oblongata,Spinal Cord and Gastric Mucosa in Depression Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 172-175. DOI： 10.13422/j.cnki.syfjx.2014240172.

摘要

目的: 观察疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃黏膜组织降钙素基因相关肽(CGRP)表达的影响

揭示疏肝和胃汤改善抑郁状态及胃肠功能的作用机制. 方法: 100只SPF级Wistar大鼠

雌雄各半

随机分成正常组、模型组、氟西汀组及疏肝和胃汤高、低剂量组.采用慢性不可预知温和应激加孤养法制作抑郁模型

共计造模4周.在第3周

氟西汀组及疏肝和胃汤高、低剂量组分别予以0.36 mg·kg-1

10 g·kg-1给药

正常组及模型组给予等体积生理盐水

每天分2次灌胃.在第4周结束后

使用内固定法取大鼠延髓迷走神经背核部位的脑、胸髓(T6-T8

根据脊神经定位)及胃黏膜组织

应用免疫组织化学技术检测上述部位CGRP的表达. 结果: 模型组大鼠延髓、脊髓中CGRP含量明显上升

胃黏膜CGRP含量明显上升

与正常组比较有非常显著性差异(P<0.01);治疗2周后

疏肝和胃汤高、低剂量组和氟西汀组大鼠延髓、脊髓组织中CGRP含量显著下降

胃黏膜CGRP含量明显减少

接近恢复至生理水平

与模型组比较有显著性差异(P<0.05

P<0.01);其中

疏肝和胃汤高剂量组与氟西汀组延髓、脊髓中CGRP含量比较无显著性差异

但胃黏膜中CGRP含量与氟西汀组相比

有显著性差异(P<0.05). 结论: 疏肝和胃汤通过降低延髓、脊髓及胃黏膜中CGRP的表达

调节“延髓-脊髓-胃”脑肠轴通路中CGRP的含量

可能是其改善抑郁状态与胃肠功能的作用机制之一.

Abstract

Objective: To observe the effect of Shugan Hewei decoction (SHD) on the expression of calcitonin gene related protein (CGRP) of medulla oblongata

spinal cord and gastric mucosa in depression model rats. Method: Totally 100 SPF Wistar rats (half male and female) were randomized into the normal group

model group

fluoxetine 0.36 mg·kg-1 group and the high and low dosage groups of SHD

with 20 in each group. The depression model was established with chronic unpredictable mild stress (CUMS) and separation for 4 weeks. The 3rd week after modeling each treatment group was given corresponding medicines. Fluoxetine group and high

low dosage(20

10 g·kg-1) of SHD group and were given for fourteen days. At the end of the fourth week

the internal fixation method was used to collect medulla oblongata vagus nerve nucleus tissue

spinal cord tissue (T6-T8) and gastric mucosa tissue. The method of immunohistochemistry (IHC) was used to detect the expression of CGRP in the above organizations. Result: Comparing with the normal group

the content of CGRP increased significantly in medulla oblongata

spinal cord and gastric mucosa of model rats(P<0.01). Compared with the model group

the content of CGRP reduced significantly in medulla oblongata

spinal cord and gastric mucosa of high

low dosage of SHD group and fluoxetine group(P<0.05

P<0.01).In addition

the effect of high dosage of SHD was equal to fluoxetine group in medulla oblongata and spinal cord

while the former was better than the later in gastric mucosa(P<0.05). Conclusion: SHD has regulation effects on the expression of CGRP in the signal path of brain-gut axis ‘medulla oblongata-spinal cord-gastric mucosa’

which may be part of the mechanism for relieving depression and improving gastrointestinal function.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Excavation of New Prescription for Chronic Atrophic Gastritis and Its Verification of Syndrome Type of Deficiency of Stomach Yin

Mahoniae Caulis Alkaloids Ameliorate Depression by Regulating Synaptic Plasticity via cAMP Pathway

Characteristic Expression of Multiple Neurotransmitters Oscillation Imbabance in Brains of 1 028 Patients with Depression

Bupleuri Radix Associated Prescriptions Against Depression： A Review

Effect of Processed Polygonatum cyrtonema in Preventing Depression Induced by Chronic Unpredictable Mild Stress in Female Rats

Related Author

SHAO Changming

WANG Hongyu

XIE Shanshan

LIN Baitong

FAN Zhengyang

WANG Fang

LIN Yuyu

ZENG Binfang

Related Institution

College of Traditional Chinese Medicine， Xinjiang Medical University

Medical Department of Integrated Traditional Chinese and Western Medicine Hospital of Xinjiang Uygur Autonomous Region

Key Laboratory of Chemistry and Engineering of Forest Products， National Ethnic Affairs Commission， Guangxi Key Laboratory of Chemistry and Engineering of Forest Products/Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products， School of Chemistry and Chemical Engineering， Guangxi Minzu University

Guangxi Key Laboratory of Chinese Medicine Quality Standard， Guangxi Institute of Chinese Medicine and Pharmaceutical Science

Guangxi Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰