Objective: To investigate role of accessories in processing of Rehmanniae Radix Praeparata with Jianchang band and to offer reference for illustrating its processing mechanisms. Method: Fingerprints of Rehmanniae Radix Praeparata was determined by HPLC

mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) for gradient elution(0-12 min

5%-10%A;12-40 min

10%-15%A;40-70 min

15%-25%A;70-85 min

25%-28%A) and detection wavelength was 320 nm

chromatogram difference of processed products was evaluated by 2004 A version of‘Chinese medicine fingerprint similarity evaluation system’. Result: There were obvious differences between Rehmanniae Radix and Rehmanniae Radix Praeparata.In contrast to Wenzhi Rehmanniae Radix Praeparata

similarity values of rice wine negative contrast

Amomi Fructus negative contrast

Citri Reticulatae Pericarpium negative contrast

Amomi Fructus and Citri Reticulatae Pericarpium negative contrast

Amomi Fructus and Citri Reticulatae Pericarpium and rice wine negative contrast were 0.844

0.741

0.727

0.717

0.717

respectively. Conclusion: This method is simple

accurate and reproducible.It demonstrates that new components are produced or contents of known components are changed due to accessories during processing of Rehmanniae Radix Praeparata.