Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma

SUN Le; LI Chun-mei; GUO Ying-ying; WU Wei; LIU Shu-ying

doi:10.13422/j.cnki.syfjx.2015010021

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Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma

更新时间：2024-01-04

- Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 21, Issue 1, Pages: 21-23(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015010021
  CLC： R284.2
- Published：2015
- 稿件说明：
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SUN Le, LI Chun-mei, GUO Ying-ying, et al. Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 21-23.
DOI：

SUN Le, LI Chun-mei, GUO Ying-ying, et al. Optimization of Extraction Process of Ginsenosides from Flowers of Ginseng Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 21-23. DOI： 10.13422/j.cnki.syfjx.2015010021.

摘要

目的: 优选人参花中总皂苷的提取工艺条件。方法: 采用UV测定总皂苷含量

以人参皂苷Re为指标成分

检测波长550 nm。以总皂苷提取量为指标

通过正交试验考察料液比、乙醇体积分数、提取时间和提取次数对人参花中总皂苷提取工艺的影响。结果: 总皂苷最佳提取工艺为料液比1:10

乙醇体积分数80%

提取温度85℃

提取时间1 h

提取数3次;总皂苷得率18.66%。结论: 优选的提取工艺稳定、合理

有效成分提取率高

为人参花资源的开发利用提供参考。

Abstract

Objective: To optimize extraction process of ginsenosides from flowers of Ginseng Radix et Rhizoma. Method: UV was adopted to determine the content of total saponins with ginsenoside Re as index ingredient and detection wavelength at 550 nm.Taking extracting amount of total saponins as index

orthogonal test was adopted to optimize extraction technology of ginsenosides with material-liquid ratio

ethanol concentration

extraction time and times as factors.The content of ginsenosides was determined by UV spectrophotometry. Result: Optimal extraction conditions were as follows:extracted thrice with 10 times the amount of 80% ethanol at 85℃

1 h for each time;yield of total saponins was 18.66%. Conclusion: This optimized extraction process is stable and reasonable with high yield of active constituents

it can provide a reference for development and utilization of flower resource of Ginseng Radix et Rhizoma.

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Related Author

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KUANG Hai-xue

HONG Kui

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Medical School， Lijiang Culture and Tourism College

College of Food and Biological Engineering，The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs， Chongqing Three Gorges University

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of Chinese Medicine

Chengdu University of TCM

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