ZHANG Hua-tan, ZHENG Wen-li, WEI Yan-ting, et al. Optimization of Purification Process of Total Flavonoids from Abelmoschi Corolla by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 28-31.
DOI:
ZHANG Hua-tan, ZHENG Wen-li, WEI Yan-ting, et al. Optimization of Purification Process of Total Flavonoids from Abelmoschi Corolla by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(1): 28-31. DOI: 10.13422/j.cnki.syfjx.2015010028.
Optimization of Purification Process of Total Flavonoids from Abelmoschi Corolla by Macroporous Resin
Objective: To optimize purification process of total flavonoids from Abelmoschi Corolla with macroporous resin. Method: Five kinds of resins for purification of total flavonoids from Abelmoschi Corolla were compared by static adsorption and elution test.UV was employed to determine the content of total flavonoids with detection wavelength at 510 nm;based on single factor tests
taking composite score of quality and purity of total flavonoids as index
orthogonal design were used to optimize purification process conditions with ethanol concentration
concentration and pH of sample solution as factors. Result: AB-8 macroporous resin was selected
optimum technological conditions were as follows:ratio of diameter-height 1:6
sample solution concentration of 0.2 g·mL-1
pH 5.0
volume of sample solution 3 BV
with 6 BV of 75% ethanol as eluant;quality and purity of total flavonoids were 417.5 mg and 58.04%. Conclusion: Total flavonoids from Abelmoschi Corolla can be effectively purificated and separated by AB-8 macroporous resin.
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Related Author
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