Objective: To optimize inclusion process of volatile oil in Shidan granules

in order to provide a reference for clinical application and promotion of this preparation. Method: With inclusion rate of volatile oil and inclusion yield as comprehensive evaluation index

effects of saturated water solution and trituration method on inclusion were compared by single factor tests.Orthogonal design was used to optimize inclusion technology with ratio of volatile oil to β-cyclodextrin(β-CD)

the amount of water and grinding time as factors

inclusion complex of volatile oil were detected by TLC

UV and microscopic imaging

then its stability was investigated. Result: Trituration method was adopted

the best inclusion conditions were as follows:β-CD-volatile oil(8:1)

triturated 3 hours with 3 times the amount of water;encapsulation efficiency of volatile oil was 87.04% and yield of inclusion complex was 89.12%.Volatile oil retention rates of β-CD inclusion complex in the zeroth

first

third day were 100%

89.23% and 78.46%. Conclusion: Volatile oil is packed into cavity of β-CD and relatively stable β-CD inclusion complex is formed

stability of Shidan granules significantly increases.This optimized technology is stable and feasible

which is suitable for large-scale production of this preparation.